Anti-UHRF1 antibody (ab57083)

Overview

• Product nameAnti-UHRF1 antibodySee all UHRF1 primary antibodies ...
• Description
  Mouse monoclonal to UHRF1
• Tested applicationsWB, IHC-P, ICC/IF, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment: WNEVLASLKD RPASGSPFQL FLSKVEETFQ CICCQELVFR PITTVCQHNV CKDCLDRSFR AQVFSCPACR YDLGRSYAMQ VNQPLQTVLN QLFPGYGNGR , corresponding to amino acids 694-794 of Human UHRF1

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

  • Epigenetics and Nuclear Signaling
  • Chromatin Binding Proteins
  • Methyl Lysine

Applications

Target

• FunctionPutative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.
• Tissue specificityExpressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
• PathwayProtein modification; protein ubiquitination.
• Sequence similaritiesContains 1 PHD-type zinc finger.
  Contains 2 RING-type zinc fingers.
  Contains 1 ubiquitin-like domain.
  Contains 1 YDG domain.
• Developmental stageExpressed in fetal thymus, liver and kidney.
• DomainThe RING finger is required for ubiquitin ligase activity.
  The YDG domain mediates the interaction with histone H3.
• Post-translational
  modificationsPhosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
  Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q96T88 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 29128 Human
  • Omim: 607990 Human
  • SwissProt: Q96T88 Human
  • Unigene: 108106 Human

• Alternative names
  Ac2-121 antibodyAL022808 antibodyE3 ubiquitin-protein ligase UHRF1 antibody

  EC 6.3.2.- antibodyFLJ21925 antibodyhNP95 antibodyhUHRF1 antibodyHuNp95 antibodyICBP90 antibodyInverted CCAAT box binding protein of 90 kDa antibodyInverted CCAAT box binding protein, 90-kD antibodyInverted CCAAT box-binding protein of 90 kDa antibodyLiver regeneration-related protein LRRG126 antibodyMGC138707 antibodyNP95 antibodyNuclear phosphoprotein, 95-KD antibodyNuclear protein 95 antibodyNuclear zinc finger protein Np95 antibodyRING finger protein 106 antibodyRNF106 antibodyTranscription factor ICBP90 antibodyUbiquitin like containing PHD and RING finger domains protein 1 antibodyUbiquitin like PHD and RING finger domain containing protein 1 antibodyUbiquitin-like PHD and RING finger domain-containing protein 1 antibodyUbiquitin-like protein containing PHD and RING finger domains 1 antibodyUbiquitin-like with PHD and ring finger domains 1 antibodyUbiquitin-like, containing PHD and RING finger domains, 1 antibodyUbiquitin-like-containing PHD and RING finger domains protein 1 antibodyUHRF1 antibodyUHRF1_HUMAN antibody

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Anti-UHRF1 antibody images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UHRF1 antibody (ab57083)
  UHRF1 antibody (ab57083) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium.
• 

  Western blot - UHRF1 antibody (ab57083)
  
  
  Predicted band size : 90 kDa
  UHRF1 antibody (ab57083) at 1ug/lane + HeLa cell lysate at 25ug/lane.
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  Immunocytochemistry/ Immunofluorescence-UHRF1 antibody(ab57083)
  ICC/IF image of ab57083 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57083, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
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  Flow Cytometry-Anti-UHRF1 antibody(ab57083)
  Overlay histogram showing MCF7 cells stained with ab57083 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57083, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.