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Products:Immunology >> Innate Immunity >> NK Cells
Overview
Product name
Anti-ULBP2 antibody
See all ULBP2 products (3) ...
Description
Mouse polyclonal to ULBP2
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length ULBP2 protein (Human)
Positive control
Human ovary cancer tissue lysate and ULBP2 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Immunology >> Innate Immunity >> NK Cells
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
WB
Application notes
WB: 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa).
Detection Antibody Pair.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ULBP2 is a human ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The interaction with UL16 blocks the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system UL16 also causes ULBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. ULBP2 is expressed in various types of cancer cell lines and in the fetus, but not in normal tissues.
Cellular localization
Cell Membrane and Secreted
Alternative names
- ALCAN alpha antibody
- N2DL 2 antibody
- N2DL2 antibody
see all
Database links
- Entrez Gene: 80328 Human
- Omim: 605698 Human
- SwissProt: Q9BZM5 Human
- Unigene: 656778 Human
Anti-ULBP2 antibody images:
Western blot - ULBP2 antibody (ab67186)
Anti-ULBP2 antibody (ab67186) at 1/500 dilution + human ovary cancer tissue lysate at 25 µg/ml
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 27 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Western blot - ULBP2 antibody (ab67186)
All lanes : Anti-ULBP2 antibody (ab67186) at 1/500 dilution
Lane 1 : ULBP2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg/ml per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 27 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
References for Anti-ULBP2 antibody (ab67186)
ab67186 has not yet been referenced specifically in any publications.
Publishing research using ab67186? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"