Anti-ULK1 antibody (ab71059)

Overview

• Product nameAnti-ULK1 antibodySee all ULK1 primary antibodies ...
• Description
  Rabbit polyclonal to ULK1
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  A KLH conjugated synthetic peptide selected from the N terminal region of human ULK1.

• Positive controlK562 cell line lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein A purified
• Purification notesThe antibody was purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitophagy fission and fusion

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Cardiovascular
  • Heart
  • Autophagy
  • Other

  • Neuroscience
  • Neurology process
  • Neurogenesis

Applications

Target

• FunctionInvolved in autophagy. Required for autophagosome formation (By similarity). Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity). Phosphorylates ATG13/KIAA0652. Involved in axon growth (By similarity). Plays an essential role in neurite extension of cerebellar granule cells.
• Tissue specificityUbiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
• Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin.
• Cellular localizationCytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.

Target information above from: UniProt accession O75385 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8408 Human
  • Omim: 603168 Human
  • SwissProt: O75385 Human
  • Unigene: 47061 Human

• Alternative names
  ATG 1 antibodyATG1 antibodyATG1 autophagy related 1 homolog antibody

  ATG1A antibodyAutophagy related protein 1 homolog antibodyFLJ38455 antibodyFLJ46475 antibodyhATG1 antibodyKIAA0722 antibodySerine/threonine protein kinase ULK1 antibodySerine/threonine protein kinase Unc51.1 antibodySerine/threonine-protein kinase ULK1 antibodyULK 1 antibodyULK1 antibodyULK1_HUMAN antibodyUnc 51 (C. elegans) like kinase 1 antibodyUNC 51 antibodyUnc 51 like kinase 1 antibodyUnc-51 like kinase 1 (C. elegans) antibodyUnc-51-like kinase 1 antibodyUNC51 antibodyUNC51, C. elegans, homolog of antibodyUnc51.1 antibody

  see all

Anti-ULK1 antibody images

• 

  Western blot - ULK1 antibody (ab71059)
  Anti-ULK1 antibody (ab71059) at 1/60 dilution + K562 cell line lysate at 35 µg
  
  Predicted band size : 113 kDa
  Observed band size : 95 kDa (why is the actual band size different from the predicted?)