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Overview

  • Product nameULK1 protein (Active)See all ULK1 proteins and peptides ...
  • Protein descriptionRecombinant fragment corresponding to amino acids 1-649 of Human ULK1 with N terminal proprietary tag; MWt 125 kDa (UniProt O75385).
  • Uniprot accessionO75385
  • Molecular weight125kDa inclusive of tags
  • Protein length649 amino acids
  • Expression hostBaculovirus infected sf9 cells

    • Properties

  • Purity> 70 % Densitometry
  • Purification notesPurity was determined to be >70% by densitometry.
  • Biological activityThe specific activity of ab125656 was determined to be 50 - 56 nmol/min/mg.
  • E.C. Number2.7.11.1
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferpH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab125656 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration.
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        ATG 1ATG1ATG1 autophagy related 1 homolog
        ATG1AAutophagy related protein 1 homologFLJ38455FLJ46475hATG1KIAA0722Serine/threonine protein kinase ULK1Serine/threonine protein kinase Unc51.1Serine/threonine-protein kinase ULK1ULK 1ULK1ULK1_HUMANUNC 51Unc 51 (C. elegans) like kinase 1Unc 51 like kinase 1Unc-51 like kinase 1 (C. elegans)Unc-51-like kinase 1UNC51UNC51, C. elegans, homolog ofUnc51.1
      see all
  • FunctionInvolved in autophagy. Required for autophagosome formation (By similarity). Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity). Phosphorylates ATG13/KIAA0652. Involved in axon growth (By similarity). Plays an essential role in neurite extension of cerebellar granule cells.
  • Tissue specificityUbiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin.
  • Cellular localizationCytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.

    • Target information above from: UniProt accession O75385 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ULK1 protein (Active) images

    • SDS-PAGE - ULK1 protein (Active) (ab125656)
      SDS-PAGE - ULK1 protein (Active) (ab125656)
      SDS-PAGE analysis of ab125656.
    • Functional Studies - ULK1 protein (Active) (ab125656)
      Functional Studies - ULK1 protein (Active) (ab125656)
      Sample Kinase Assay showing the specific activity of ab125656 as 50 nmol/min/mg.

    References for ULK1 protein (Active) (ab125656)

    ab125656 has not yet been referenced specifically in any publications.

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