Anti-UMP/CMP kinase antibody (ab77457)

Overview

• Product nameAnti-UMP/CMP kinase antibodySee all UMP/CMP kinase primary antibodies ...
• Description
  Mouse monoclonal to UMP/CMP kinase
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length protein, corresponding to amino acids 1 - 228 of Human UMP/CMP kinase (AAH14961), with a 26 kDa tag

• Positive controlK-562 cell lysate; immunogen

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Nucleotide metabolism
  • Purine and pyrimidine synthesis

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA / Nucleotides

Applications

Target

• FunctionCatalyzes specific phosphoryl transfer from ATP to UMP and CMP.
• Tissue specificityUbiquitously expressed.
• Sequence similaritiesBelongs to the adenylate kinase family.
• Cellular localizationNucleus. Cytoplasm. Mainly nuclear.

Target information above from: UniProt accession P30085 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 51727 Human
  • Omim: 191710 Human
  • SwissProt: P30085 Human
  • Unigene: 727554 Human

• Alternative names
  CMK antibodyCMPK 1 antibodyCMPK antibody

  cmpk1 antibodyCytidine monophosphate (UMP CMP) kinase 1 cytosolic antibodyCytidine monophosphate kinase antibodyCytidine monophosphate UMP CMP kinase 1 cytosolic antibodyCytidylate kinase antibodyDeoxycytidylate kinase antibodyEC 2.7.4.14 antibodyKCY antibodyKCY_HUMAN antibodyOTTHUMP00000009565 antibodyRP11 511I2.1 antibodyUCK antibodyUMK antibodyUMP CMP antibodyUMP CMP kinase antibodyUMP-CMP kinase antibodyUMP/CMP kinase antibodyUMP/CMPK antibodyUMPK antibodyUridine monophosphate kinase antibodyUridine monophosphate/cytidine monophosphate kinase antibody

  see all

Anti-UMP/CMP kinase antibody images

• 

  Western blot - UMP/CMP kinase antibody (ab77457)
  Anti-UMP/CMP kinase antibody (ab77457) at 1/500 dilution + K-562 cell lysate at 25 µg
  
  Secondary
  goat anti-mouse IgG (H&L)- HRP conjugate at 1/2500 dilution
  
  Predicted band size : 22 kDa
  Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - UMP/CMP kinase antibody (ab77457)
  Anti-UMP/CMP kinase antibody (ab77457) at 1/500 dilution + recombinant tagged UMP/CMP kinase protein (the immunogen) at 0.2 µg
  
  Secondary
  goat anti-mouse IgG (H&L)- HRP conjugate at 1/5000 dilution
  
  Predicted band size : 22 kDa
  Observed band size : 51 kDa (why is the actual band size different from the predicted?)