Anti-Nucleoside-diphosphate kinase antibody (ab225940)
Key features and details
- Rabbit polyclonal to Nucleoside-diphosphate kinase
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nucleoside-diphosphate kinase antibody
See all Nucleoside-diphosphate kinase primary antibodies -
Description
Rabbit polyclonal to Nucleoside-diphosphate kinase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human Nucleoside-diphosphate kinase aa 1 to the C-terminus.
Database link: P30085-1 -
Positive control
- WB: HEK-293T and HeLa whole cell lysates. IHC-P: Human colon cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. Predicted molecular weight: 22 kDa. |
Target
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Function
Catalyzes specific phosphoryl transfer from ATP to UMP and CMP. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the adenylate kinase family. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 509965 Cow
- Entrez Gene: 51727 Human
- Entrez Gene: 66588 Mouse
- Entrez Gene: 100521561 Pig
- Entrez Gene: 298410 Rat
- Omim: 191710 Human
- SwissProt: Q2KIW9 Cow
- SwissProt: P30085 Human
see all -
Alternative names
- CMK antibody
- CMPK 1 antibody
- CMPK antibody
see all
Images
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Paraffin-embedded human colon cancer tissue stained for Nucleoside-diphosphate kinase using ab225940 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-Nucleoside-diphosphate kinase antibody (ab225940) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab225940 has been referenced in 1 publication.
- Chu H et al. CMPK1 Regulated by miR-130b Attenuates Response to 5-FU Treatment in Gastric Cancer. Front Oncol 11:637470 (2021). PubMed: 33816278