Anti-UNG antibody [2F3] (ab118977)

Overview

• Product nameAnti-UNG antibody [2F3]See all UNG primary antibodies ...
• Description
  Mouse monoclonal [2F3] to UNG
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length Human UNG protein (NP_550433).

• Positive controlHEK293T cell lysate transfected with pCMV6-ENTRY UNG cDNA. COS7 cells transiently transfected by pCMV6-ENTRY UNG
• General notesDilute in PBS (pH7.3) before use.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 1% BSA, 50% Glycerol
• Concentration information loading...
• PurityProtein A purified
• Purification notesPurified from mouse ascites fluids by affinity chromatography.
• Clonality Monoclonal
• Clone number2F3
• IsotypeIgG2a
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Base Excision Repair

Applications

Target

• FunctionExcises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
• Tissue specificityIsoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
• Involvement in diseaseDefects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
• Sequence similaritiesBelongs to the uracil-DNA glycosylase family.
• Post-translational
  modificationsIsoform 1 is processed by cleavage of a transit peptide.
• Cellular localizationMitochondrion and Nucleus.

Target information above from: UniProt accession P13051 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7374 Human
  • Omim: 191525 Human
  • SwissProt: P13051 Human
  • Unigene: 191334 Human

• Alternative names
  DGU antibodyDKFZp781L1143 antibodyHIGM 4 antibody

  HIGM4 antibodyOTTHUMP00000240527 antibodyOTTHUMP00000240528 antibodyOTTHUMP00000240529 antibodyUDG antibodyUNG 1 antibodyUNG 15 antibodyung antibodyUNG_HUMAN antibodyUNG1 antibodyUNG15 antibodyUNG2 antibodyUracil DNA glycosylase 1 antibodyUracil DNA glycosylase 2 antibodyUracil DNA glycosylase antibody

  see all

Anti-UNG antibody [2F3] images

• 

  Western blot - Anti-UNG antibody [2F3] (ab118977)
  All lanes : Anti-UNG antibody [2F3] (ab118977) at 1/2000 dilution
  
  Lane 1 : HEK293T cells were transfected with the pCMV6-ENTRY control
  Lane 2 : HEK293T cells were transfected with the pCMV6-ENTRY UNG cDNA
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 35 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-UNG antibody [2F3] (ab118977)
  Anti-UNG mouse monoclonal antibody (ab118977) immunofluorescent staining of COS7 cells transiently transfected with pCMV6-ENTRY UNG cDNA.