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- Secondary antibodies
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Product name
Anti-UNG antibody
See all UNG products (11) ...
Description
Rabbit polyclonal to UNG
Tested applications
Cross reactivity
Predicted to work with
Mouse, Rat, Chicken, Human, Zebrafish
Immunogen
Synthetic peptide: CRHFSKTNELLQKSGKKP, corresponding to amino acids 281-298 of Human UNG.
CRHFSKTNELLQKSGKKP
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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Western blot - UNG antibody (ab13668)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 36.5 kDa).
Target
Function
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
Tissue specificity
Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
Involvement in disease
Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
Sequence similarities
Belongs to the uracil-DNA glycosylase family.
Post-translational
modifications
Isoform 1 is processed by cleavage of a transit peptide.
Cellular localization
Mitochondrion and Nucleus.
Target information above from: UniProt accessionP13051
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DGU antibody
- DKFZp781L1143 antibody
- HIGM 4 antibody
see all
Database links
- Entrez Gene: 7374 Human
- Entrez Gene: 22256 Mouse
- Entrez Gene: 304577 Rat
- Omim: 191525 Human
- SwissProt: P13051 Human
- SwissProt: P97931 Mouse
- Unigene: 191334 Human
- Unigene: 1393 Mouse
see all
Anti-UNG antibody images:
Western blot - UNG antibody (ab13668)
Predicted band size : 36.5 kDa
Detection of UNG in HeLa cell lysate with anti-UNG ab13668 at 2µg/ml dilution.
References for Anti-UNG antibody (ab13668)
This product has been referenced in:
- Pauklin S et al. Alternative induction of meiotic recombination from single-base lesions of DNA deaminases. Genetics 182:41-54 (2009).Read more (PubMed: 19237686) »
- Di Noia JM et al. Dependence of antibody gene diversification on uracil excision. J Exp Med 204:3209-19 (2007).Read more (PubMed: 18070939) »
See all 3 publications for this product
Publishing research using ab13668? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"