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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Overview
Product name
Anti-UNG antibody
See all UNG products (11) ...
Description
Rabbit polyclonal to UNG
Tested applications
WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human UNG.
(Peptide available as ab25856.)
Positive control
In WB, this antibody gave a positive signal in HeLa and Jurkat whole cell lysates, and in HeLa nuclear lysate. In IF, we observed positive staining in HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 35, 39 kDa representing two alternatively spliced forms of UNG that are differentially localized (predicted molecular weight: 33, 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
Tissue specificity
Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
Involvement in disease
Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
Sequence similarities
Belongs to the uracil-DNA glycosylase family.
Post-translational
modifications
Isoform 1 is processed by cleavage of a transit peptide.
Cellular localization
Mitochondrion and Nucleus.
Target information above from: UniProt accessionP13051
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DGU antibody
- DKFZp781L1143 antibody
- HIGM 4 antibody
see all
Database links
- Entrez Gene: 7374 Human
- Entrez Gene: 22256 Mouse
- Entrez Gene: 304577 Rat
- Omim: 191525 Human
- SwissProt: P13051 Human
- SwissProt: P97931 Mouse
- Unigene: 191334 Human
- Unigene: 1393 Mouse
see all
Anti-UNG antibody images:
Western blot - UNG antibody (ab23926)
All lanes : Anti-UNG antibody (ab23926) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : HeLa (Human epithelial carcinoma cell line) nuclear lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Predicted band size : 33, 35 kDa
Observed band size : 35,39 kDa (why is the actual band size different from the predicted?)
Additional bands at : 18 kDa (possible cleavage fragment,cross reactivity),23 kDa (possible cleavage fragment,cross reactivity),40 kDa (possible cross reactivity).
UNG is present in the cell in two isoforms, that differ at their amino-terminal ~40 amino acids. This results in one isoform that is present in the nucleus and one that is present in the mitochondria. This antibody strongly recognizes a doublet at approximately 35 and 39 kDa which closely correspond in size to these two isoforms (whose predicted molecular weights are 33 and 35 kDa).
Immunocytochemistry/ Immunofluorescence - UNG antibody (ab23926)
ICC/IF image of ab23926stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23926, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UNG antibody (ab23926)
Image courtesy of Human Protein Atlas
ab23926 staining UNG in human heart muscle. Paraffin embedded human heart muscle tissue was incubated with ab23926 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab23926 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
References for Anti-UNG antibody (ab23926)
This product has been referenced in:
- Langevin Cet al. Human immunodeficiency virus type 1 Vpr modulates cellular expression of UNG2 via a negative transcriptional effect. J Virol 83:10256-63 (2009). WB; Human.Read more (PubMed: 19625402) »
- Guo Cet al. Ugene, a newly identified protein that is commonly overexpressed in cancer and binds uracil DNA glycosylase. Cancer Res 68:6118-26 (2008). WB; Human.Read more (PubMed: 18676834) »
See all 3 publications for this product
Publishing research using ab23926? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"