Overview

  • Product nameAnti-UNG antibody
    See all UNG primary antibodies
  • Description
    Rabbit polyclonal to UNG
  • Tested applicationsWB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human UNG.

    (Peptide available as ab25856.)

  • Positive control
    • In WB, this antibody gave a positive signal in HeLa and Jurkat whole cell lysates, and in HeLa nuclear lysate. In IF, we observed positive staining in HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab23926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-P
  • Application notesICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 35, 39 kDa representing two alternatively spliced forms of UNG that are differentially localized (predicted molecular weight: 33, 35 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionExcises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
    • Tissue specificityIsoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
    • Involvement in diseaseDefects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
    • Sequence similaritiesBelongs to the uracil-DNA glycosylase family.
    • Post-translational
      modifications
      Isoform 1 is processed by cleavage of a transit peptide.
    • Cellular localizationMitochondrion and Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DGU antibody
      • DKFZp781L1143 antibody
      • HIGM 4 antibody
      • HIGM4 antibody
      • OTTHUMP00000240527 antibody
      • OTTHUMP00000240528 antibody
      • OTTHUMP00000240529 antibody
      • UDG antibody
      • UNG 1 antibody
      • UNG 15 antibody
      • ung antibody
      • UNG_HUMAN antibody
      • UNG1 antibody
      • UNG15 antibody
      • UNG2 antibody
      • Uracil DNA glycosylase 1 antibody
      • Uracil DNA glycosylase 2 antibody
      • Uracil DNA glycosylase antibody
      • Uracil-DNA glycosylase antibody
      see all

    Anti-UNG antibody images

    • All lanes : Anti-UNG antibody (ab23926) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
      Lane 3 : HeLa (Human epithelial carcinoma cell line) nuclear lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

      Predicted band size : 33, 35 kDa
      Observed band size : 35,39 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 18 kDa (possible cleavage fragment,cross reactivity),23 kDa (possible cleavage fragment,cross reactivity),40 kDa (possible cross reactivity).
    • ICC/IF image of ab23926stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23926, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

    • Image courtesy of Human Protein Atlas

      ab23926 staining UNG in human heart muscle. Paraffin embedded human heart muscle tissue was incubated with ab23926 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

      ab23926 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

      Further results for this antibody can be found at www.proteinatlas.org

    References for Anti-UNG antibody (ab23926)

    This product has been referenced in:
    • Kitamura K  et al. Uracil DNA glycosylase counteracts APOBEC3G-induced hypermutation of hepatitis B viral genomes: excision repair of covalently closed circular DNA. PLoS Pathog 9:e1003361 (2013). WB ; Human . Read more (PubMed: 23696735) »
    • Bulgar AD  et al. Removal of uracil by uracil DNA glycosylase limits pemetrexed cytotoxicity: overriding the limit with methoxyamine to inhibit base excision repair. Cell Death Dis 3:e252 (2012). ICC/IF ; Human . Read more (PubMed: 22237209) »

    See all 6 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"