Anti-UNG antibody [k1C12] (ab58976)
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberk1C12
- Light chain typekappa
- Research Areas
Our Abpromise guarantee covers the use of ab58976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||ELISA: Use at an assay dependent dilution.|
|WB||WB: 1/1000 - 1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).|
- FunctionExcises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
- Tissue specificityIsoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
- Involvement in diseaseDefects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
- Sequence similaritiesBelongs to the uracil-DNA glycosylase family.
modificationsIsoform 1 is processed by cleavage of a transit peptide.
- Cellular localizationMitochondrion and Nucleus.
- DGU antibodyDKFZp781L1143 antibodyHIGM 4 antibody
- HIGM4 antibodyOTTHUMP00000240527 antibodyOTTHUMP00000240528 antibodyOTTHUMP00000240529 antibodyUDG antibodyUNG 1 antibodyUNG 15 antibodyung antibodyUNG_HUMAN antibodyUNG1 antibodyUNG15 antibodyUNG2 antibodyUracil DNA glycosylase 1 antibodyUracil DNA glycosylase 2 antibodyUracil DNA glycosylase antibodyUracil-DNA glycosylase antibody
Anti-UNG antibody [k1C12] images
Anti-UNG antibody [k1C12] (ab58976) at 1/1000 dilution + HeLa cell lysate at 30 µg
HRP-conjugated goat anti-mouse
developed using the ECL technique
Predicted band size : 35 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
References for Anti-UNG antibody [k1C12] (ab58976)
ab58976 has not yet been referenced specifically in any publications.