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Overview

  • Product nameAnti-UNG antibody [k1C12]See all UNG primary antibodies ...
  • Description
    Mouse monoclonal [k1C12] to UNG
  • Tested applicationsELISA, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human full length protein purified from E. coli.

  • Positive controlHeLa cells.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone numberk1C12
  • MyelomaSp2/0
  • IsotypeIgG2b
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab58976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use at an assay dependent dilution.
WB WB: 1/1000 - 1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).

Target

  • FunctionExcises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
  • Tissue specificityIsoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
  • Involvement in diseaseDefects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
  • Sequence similaritiesBelongs to the uracil-DNA glycosylase family.
  • Post-translational
    modifications    Isoform 1 is processed by cleavage of a transit peptide.
  • Cellular localizationMitochondrion and Nucleus.

    • Target information above from: UniProt accession P13051 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        DGU antibodyDKFZp781L1143 antibodyHIGM 4 antibody
        HIGM4 antibodyOTTHUMP00000240527 antibodyOTTHUMP00000240528 antibodyOTTHUMP00000240529 antibodyUDG antibodyUNG 1 antibodyUNG 15 antibodyung antibodyUNG_HUMAN antibodyUNG1 antibodyUNG15 antibodyUNG2 antibodyUracil DNA glycosylase 1 antibodyUracil DNA glycosylase 2 antibodyUracil DNA glycosylase antibodyUracil-DNA glycosylase antibody
      see all

    Anti-UNG antibody [k1C12] images

    • Western blot - UNG antibody [k1C12] (ab58976)
      Western blot - UNG antibody [k1C12] (ab58976)
      Anti-UNG antibody [k1C12] (ab58976) at 1/1000 dilution + HeLa cell lysate at 30 µg

      Secondary
      HRP-conjugated goat anti-mouse
      developed using the ECL technique

      Predicted band size : 35 kDa
      Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    References for Anti-UNG antibody [k1C12] (ab58976)

    ab58976 has not yet been referenced specifically in any publications.

    Publishing research using ab58976 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"