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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-UPF3B/RENT3B antibody
See all UPF3B/RENT3B products (5) ...
Description
Rabbit polyclonal to UPF3B/RENT3B
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to a region within the internal sequence amino acids 251-300 (KIDRIPERDK LKDEPKIKVH RFLLQAVNQK NLLKKPEKGD EKELDKREKA) of Human UPF3B/RENT3B (NP_542199)
KIDRIPERDK LKDEPKIKVH RFLLQAVNQK NLLKKPEKGD EKELDKREKA
Positive control
Fetal heart lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Western blot - UPF3B/RENT3B antibody (ab83249)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay 1/312500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 57 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. UPF3B binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X (referenced from entrez gene).
Cellular localization
Nucleus. Cytoplasm. Note: Shuttling between the nucleus and the cytoplasm.
Alternative names
- HUPF3B antibody
- hUpf3p X antibody
- Nonsense mRNA reducing factor 3B antibody
see all
Database links
- Entrez Gene: 65109 Human
- Entrez Gene: 68134 Mouse
- Entrez Gene: 313449 Rat
- Omim: 300298 Human
- SwissProt: Q9BZI7 Human
- SwissProt: Q149I6 Mouse
- SwissProt: Q3ULL6 Mouse
- SwissProt: D3ZBE8 Rat
Anti-UPF3B/RENT3B antibody images:
Western blot - UPF3B/RENT3B antibody (ab83249)
Anti-UPF3B/RENT3B antibody (ab83249) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 57 kDa
Observed band size : 64 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
References for Anti-UPF3B/RENT3B antibody (ab83249)
ab83249 has not yet been referenced specifically in any publications.
Publishing research using ab83249? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"