Alexa Fluor® 647 Anti-UQCRC2 antibody [13G12AF12BB11] (ab197652)
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [13G12AF12BB11] to UQCRC2
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 647 Anti-UQCRC2 antibody [13G12AF12BB11]
See all UQCRC2 primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [13G12AF12BB11] to UQCRC2 -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- ICC/IF: MEF1 cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
13G12AF12BB11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
This product gave a positive signal in MEF1 cells fixed with 100% methanol (5 min). |
Notes |
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ICC/IF
1/100. This product gave a positive signal in MEF1 cells fixed with 100% methanol (5 min). |
Target
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Function
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex. -
Sequence similarities
Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 67003 Mouse
- SwissProt: Q9DB77 Mouse
- Unigene: 334206 Mouse
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Alternative names
- Complex III subunit 2 antibody
- Core protein II antibody
- Cytochrome b c1 complex subunit 2 mitochondrial antibody
see all
Images
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ab197652 staining UQCRC2 in MEF1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197652 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab197652 has not yet been referenced specifically in any publications.