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Western blot - USF1 antibody (ab66368)
(enlarge)
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Product Name
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USF1 antibody
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See all USF1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to USF1
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Immunogen
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Synthetic peptide: QSAATFPDPN VKYVFRTENG GQVMYRVIQV SEGQLDGQTE GTGAISGYPA, corresponding to a region within N terminal amino acids 37-86 of Human USF1
BLAST 'QSAATFPDPN VKYVFRTENG GQVMYRVIQV SEGQLDGQTE GTGAISGYPA ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat, Dog
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/312500.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
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Relevance
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USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Lyophilised
Add 100 µl of distilled water for a final concentration of 1mg/ml.
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this USF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for USF1 antibody images, references, products related to ab66368 and other tools.
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USF1 antibody images:
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 Western blot - USF1 antibody (ab66368)
USF1 antibody (ab66368) at 1.25 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer
Secondary
HRP conjugated anti-Rabbit IgGat 1/50,000 - 1/100,000 dilution
Predicted band size : 34 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to USF1
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USF1 antibody - more information
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Customer reviews (feedback) regarding USF1 antibody |
Customer FAQs regarding USF1 antibody |
Protocols for USF1 antibody |
Price and availability of products related to USF1 antibody |
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USF1 antibody - related products:
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USF1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about USF1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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