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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper
Overview
Product name
Anti-USF1 antibody
See all USF1 products (6) ...
Description
Rabbit polyclonal to USF1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Pig
Immunogen
Synthetic peptide: QSAATFPDPN VKYVFRTENG GQVMYRVIQV SEGQLDGQTE GTGAISGYPA, corresponding to a region within N terminal amino acids 37-86 of Human USF1
QSAATFPDPN VKYVFRTENG GQVMYRVIQV SEGQLDGQTE GTGAISGYPA
Positive control
Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper
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Western blot - USF1 antibody (ab66368)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/312500.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this.
Cellular localization
Nuclear
Alternative names
- bHLHb11 antibody
- Class B basic helix-loop-helix protein 11 antibody
- FCHL 1 antibody
see all
Database links
- Entrez Gene: 7391 Human
- Entrez Gene: 22278 Mouse
- Entrez Gene: 83586 Rat
- Omim: 191523 Human
- SwissProt: P22415 Human
- SwissProt: Q61069 Mouse
- SwissProt: O35410 Rat
- Unigene: 414880 Human
see all
Anti-USF1 antibody images:
Western blot - USF1 antibody (ab66368)
Anti-USF1 antibody (ab66368) at 1.25 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer
Secondary
HRP conjugated anti-Rabbit IgGat 1/50,000 - 1/100,000 dilution
Predicted band size : 34 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
References for Anti-USF1 antibody (ab66368)
ab66368 has not yet been referenced specifically in any publications.
Publishing research using ab66368? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"