Recombinant Anti-USP11 antibody [EPR4346] (ab109232)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4346] to USP11
- Suitable for: WB, IP, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-USP11 antibody [EPR4346]
See all USP11 primary antibodies -
Description
Rabbit monoclonal [EPR4346] to USP11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cyt (Intra)more details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T, Jurkat, Human testis, Human fetal kidney and LnCaP cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4346 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
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IP |
1/10 - 1/300.
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Flow Cyt (Intra) |
1/500 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
IP
1/10 - 1/300. |
Flow Cyt (Intra)
1/500 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks. -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 1 DUSP domain. -
Cellular localization
Nucleus. Cytoplasm. Predominantly nuclear. Associates with chromatin. - Information by UniProt
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Database links
- Entrez Gene: 8237 Human
- Entrez Gene: 236733 Mouse
- Entrez Gene: 408217 Rat
- Omim: 300050 Human
- SwissProt: P51784 Human
- SwissProt: Q99K46 Mouse
- Unigene: 171501 Human
- Unigene: 34489 Mouse
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Alternative names
- Deubiquitinating enzyme 11 antibody
- Ubiquitin carboxyl-terminal hydrolase 11 antibody
- Ubiquitin carboxyl-terminal hydrolase X linked antibody
see all
Images
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: USP11 knockout HAP1 cell lysate (20 µg)
Lane 3: LNCaP cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab109232 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109232 was shown to specifically react with USP11 when USP11 knockout samples were used. Wild-type and USP11 knockout samples were subjected to SDS-PAGE. ab109232 and ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging. -
Overlay histogram showing Jurkat cells fixed in 4% PFA and stained with purified ab109232 at a dilution of 1 in 950 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
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ab109232 (purified) at 1/300 immunoprecipitating USP11 in 10 μg HEK293 (Lanes 1 and 2, observed at 110 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/5000 dilution (purified)
Lane 1 : mouse brain lysate
Lane 2 : rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1000 µg
Predicted band size: 110 kDa
Observed band size: 110 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling USP11 with unpurified ab109232 at 1/570 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).
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ab109232 (purified) at 1/300 immunoprecipitating USP11 in 10 μg Jurkat (Lanes 1 and 2, observed at 110 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/5000 dilution (purified)
Lane 1 : HEK293 lysate at 20 µg
Lane 2 : human fetal kidney lysate
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/1000 dilution (purified)
Lane 1 : Jurkat lysate
Lane 2 : human testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Human testis cell lysate
Lane 4 : Huma fetal kidney cell lysate
Lane 5 : LnCaP cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab109232 has been referenced in 12 publications.
- Doherty LM et al. Integrating multi-omics data reveals function and therapeutic potential of deubiquitinating enzymes. Elife 11:N/A (2022). PubMed: 35737447
- Zhang W et al. Circ_DOCK1 regulates USP11 through miR-132-3p to control colorectal cancer progression. World J Surg Oncol 19:67 (2021). PubMed: 33685455
- Zhang X et al. A pro-inflammatory mediator USP11 enhances the stability of p53 and inhibits KLF2 in intracerebral hemorrhage. Mol Ther Methods Clin Dev 21:681-692 (2021). PubMed: 34141823
- Ramirez J et al. A Proteomic Approach for Systematic Mapping of Substrates of Human Deubiquitinating Enzymes. Int J Mol Sci 22:N/A (2021). PubMed: 34063716
- Park MK et al. PTEN self-regulates through USP11 via the PI3K-FOXO pathway to stabilize tumor suppression. Nat Commun 10:636 (2019). PubMed: 30733438