Overview

  • Product name
  • Description
    Goat polyclonal to USP16
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: GKKRTKGKTVPIDD, corresponding to N terminal amino acids 2-15 of Human USP16.

  • General notes
    Gene Ontology terms - ubiquitin-specific protease activity; cysteine-type endopeptidase activity; cell cycle; ubiquitin-dependent protein catabolism; cytoplasm; ubiquitin C-terminal hydrolase activity; hydrolase activity.

Properties

Applications

Our Abpromise guarantee covers the use of ab4853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    Specifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-10' of histone H3, and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.
  • Tissue specificity
    Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in disease
    Note=A chromosomal aberration involving USP16 is a cause of Chronic myelomonocytic leukemia. Inversion inv(21) (q21;q22) with RUNX1/AML1.
  • Sequence similarities
    Belongs to the peptidase C19 family. USP16 subfamily.
    Contains 1 UBP-type zinc finger.
  • Domain
    The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.
  • Post-translational
    modifications
    Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. The phosphorylated form of the protein is also enzymatically active.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 16 antibody
    • Human ubiquitin processing protease antibody
    • MSTP039 antibody
    • Ubiquitin carboxyl-terminal hydrolase 16 antibody
    • Ubiquitin Specific peptidase 16 antibody
    • Ubiquitin Specific Protease 16 antibody
    • Ubiquitin thiolesterase 16 antibody
    • Ubiquitin-processing protease UBP-M antibody
    • Ubiquitin-specific-processing protease 16 antibody
    • UBP M antibody
    • UBP16_HUMAN antibody
    • UBPM antibody
    • usp16 antibody
    see all

Images

  • ab4853 (4µg/ml) staining of paraffin embedded Human Human Kidney following steamed antigen retrieval with Tris/EDTA buffer pH 9 and HRP-staining shows textured cytoplasm staining in the convoluted tubules.

References

ab4853 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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