Anti-USP30 antibody [CL4438] (ab219969)
Key features and details
- Mouse monoclonal [CL4438] to USP30
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-USP30 antibody [CL4438]
See all USP30 primary antibodies -
Description
Mouse monoclonal [CL4438] to USP30 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human USP30 aa 250-450.
Database link: Q70CQ3 -
Epitope
Binds to an epitope located within the peptide sequence GHKPSQHNPK as determined by overlapping synthetic peptides. -
Positive control
- Human testis, kidney, placenta and cerebellum tissues; USP30 over-expression lysate (co-expressed with myc-DDK tag, 3.1 KDa) in HEK293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL4438 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/500 - 1/1000. Predicted molecular weight: 59 kDa.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Predicted molecular weight: 59 kDa. |
Target
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Function
May participate in the maintenance of mitochondrial morphology. -
Tissue specificity
Expressed in skeletal muscle, pancreas, liver and kidney. -
Sequence similarities
Belongs to the peptidase C19 family. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 84749 Human
- Omim: 612492 Human
- SwissProt: Q70CQ3 Human
- Unigene: 486434 Human
- Unigene: 660996 Human
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Alternative names
- Deubiquitinating enzyme 30 antibody
- FLJ40511 antibody
- Ub-specific protease 30 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human testis tissue labeling USP30 with ab219969 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling USP30 with ab219969 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling USP30 with ab219969 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling USP30 with ab219969 at 1/200 dilution.
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All lanes : Anti-USP30 antibody [CL4438] (ab219969) at 1/200 dilution
Lane 1 : Control
Lane 2 : USP30 over-expression lysate (co-expressed with myc-DDK tag, 3.1 KDa) in HEK293T cells
Predicted band size: 59 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab219969 has not yet been referenced specifically in any publications.