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Overview

  • Product nameUSP48 protein (Tagged-His Tag)See all USP48 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 789-1035 (247 amino acids) of Human USP48 with N terminal His tag; Predicted MWt 29 kDa (UniProt Q86UV5).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 137 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesTKEDSKLIALIWPSEWQMIQKLFVVDHVIKITRIEVGDVN PSETQYISEPK LCPECREGLLCQQQRDLREYTQATIYV HKVVDNKKVMKDSAPELNVSSSET EEDKEEAKPDGEKD PDFNQSNGGTKRQKISHQNYIAYQKQVIRRSMRHRKV R GEKALLVSANQTLKELKIQIMHAFSVAPFDQNLSIDGKIL SDDCATLGTL GVIPESVILLKADEPIADYAAMDDVMQV CMPEEGFKGTGLLGH
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92047 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        AAH67261Deubiquitinating enzyme 48DKFZp762M1713
        EC 3.1.2.15Kiaa4202MGC132556MGC14879RAP1GA1Synaptic ubiquitin specific proteasesynUSPUbiquitin carboxyl terminal hydrolase 48Ubiquitin carboxyl-terminal hydrolase 48Ubiquitin specific peptidase 48Ubiquitin specific processing protease 48Ubiquitin specific protease 31Ubiquitin specific protease 48Ubiquitin thioesterase 48Ubiquitin thiolesterase 48Ubiquitin-specific-processing protease 48UBP48_HUMANUSP31USP48
      see all
  • FunctionRecognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at post-synaptic sites.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the peptidase C19 family.
    Contains 3 DUSP domains.
    Contains 1 ubiquitin-like domain.
  • Cellular localizationCytoplasm. Nucleus.

    • Target information above from: UniProt accession Q86UV5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    USP48 protein (Tagged-His Tag) images

    • SDS-PAGE - USP48 protein (Tagged-His Tag) (ab92047)
      SDS-PAGE - USP48 protein (Tagged-His Tag) (ab92047)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for USP48 protein (Tagged-His Tag) (ab92047)

    ab92047 has not yet been referenced specifically in any publications.

    Publishing research using ab92047 ? Please let us know so that we can cite the reference in this datasheet

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