USP48 protein (Tagged-His Tag) (ab92047)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Our Abpromise guarantee covers the use of ab92047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- AAH67261Deubiquitinating enzyme 48DKFZp762M1713
- EC 126.96.36.199Kiaa4202MGC132556MGC14879RAP1GA1Synaptic ubiquitin specific proteasesynUSPUbiquitin carboxyl terminal hydrolase 48Ubiquitin carboxyl-terminal hydrolase 48Ubiquitin specific peptidase 48Ubiquitin specific processing protease 48Ubiquitin specific protease 31Ubiquitin specific protease 48Ubiquitin thioesterase 48Ubiquitin thiolesterase 48Ubiquitin-specific-processing protease 48UBP48_HUMANUSP31USP48
Contains 3 DUSP domains.
Contains 1 ubiquitin-like domain.
USP48 protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
References for USP48 protein (Tagged-His Tag) (ab92047)
ab92047 has not yet been referenced specifically in any publications.