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Immunohistochemistry using ab12200 (C-terminus) and ab12199 (N-terminus) with peptide blocking (right panel) and without peptide blocking (left panel).
Immunofluorescence was used to detect UBE1L protein expression in MDA-MB-231 cells (red staining) and DAPI staining revealed the nuclei (blue staining). The right panel shows immunofluorescence with no primary antibody.
This picture was kindly supplied as part of the review submitted by Sonia del Rincon.
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