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Overview
Product name
Anti-Ube1L antibody
See all Ube1L products (6) ...
Description
Rabbit polyclonal to Ube1L
Specificity
The specificity of this antibody has been confirmed by immunoblot analyses of Ube1L-transfected CHO cells. CHO cells that did not basally express Ube1L mRNA were transfected with a full-length Ube1L cDNA or an insertless vector. CHO cells transfected with Ube1L expressed Ube1L, whereas cells transfected with an insertless vector did not. This antibody reacts with the N-terminus of Ube1L.
Tested applications
IHC-P, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Synthetic peptide: DCDPRSIHVREDGSLEIGD, corresponding to amino acids 222-240 of Human Ube1L.
DCDPRSIHVR EDGSLEIGD
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Ubiquitin like Modifiers
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/1000
IHC-Fr
IHC-Fr: 1/1000IHC-Fr: 1/1000
WB: 1/500Detects a band of approximately 112 kDa.
Target
Function
Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
Tissue specificity
Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the ubiquitin-activating E1 family.
Target information above from: UniProt accessionP41226
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- D8 antibody
- MGC12713 antibody
- UBA7 antibody
see all
Database links
- Entrez Gene: 7318 Human
- Omim: 191325 Human
- SwissProt: P41226 Human
- Unigene: 16695 Human
Anti-Ube1L antibody images:
Immunohistochemistry (Frozen sections) - Ube1L antibody (ab12199)
Immunohistochemistry using ab12200 (C-terminus) and ab12199 (N-terminus) with peptide blocking (right panel) and without peptide blocking (left panel).
Immunocytochemistry/ Immunofluorescence - Ube1L antibody (ab12199)
Immunofluorescence was used to detect UBE1L protein expression in MDA-MB-231 cells (red staining) and DAPI staining revealed the nuclei (blue staining). The right panel shows immunofluorescence with no primary antibody.
This picture was kindly supplied as part of the review submitted by Sonia del Rincon.
References for Anti-Ube1L antibody (ab12199)
This product has been referenced in:
- Chan EYet al. Quantitative analysis of human immunodeficiency virus type 1-infected CD4+ cell proteome: dysregulated cell cycle progression and nuclear transport coincide with robust virus production. J Virol 81:7571-83 (2007). WB; Human.Read more (PubMed: 17494070) »
- Pitha-Rowe Iet al. Involvement of UBE1L in ISG15 Conjugation during Retinoid-induced Differentiation of Acute Promyelocytic Leukemia. J Biol Chem 279:18178-87 (2004).Read more (PubMed: 14976209) »
See all 3 publications for this product
Publishing research using ab12199? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"