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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Overview
Product name
Ube2B protein (His tag)
See all Ube2B products (2) ...
Protein description
Recombinant full length Human Ube2B containing 166 amino acids including an N-terminal 6xHis tag, 19 kDa (SwissProt P63146).
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Purification notes
ab73536 is purified by proprietary chromatographic techniques. Purity greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form
Lyophilised:
Reconstitute the lyophilized Ube2B in sterile water at not less than 100µg/ml, which can then be further diluted to other aqueous solutions
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1mM DTT, 1X PBS, pH 7.5
Concentration
Concentration information loading...
Additional notes
Lyophilized Ube2B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Sequence notes
MHHHHHHAMG QLRSMSTPAR RRLMRDFKRL QEDPPVGVSG APSENNIMQW NAV IFGPEGTPFE DGTFKLVIEF SEEYPNKPPT VRFLSKMFHP NVYADGSICL DILQNRW S PTYDVSSILT SIQSLLDEPN PNSPANSQAA QLYQENKREY EKRVSAIVEQ SWNDS
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> E2 Ubiquitin Conjugating Enzymes
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab73536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Cellular localization
Cell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.
Target information above from: UniProt accessionP63146
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for Ube2B protein (His tag) (ab73536)
ab73536 has not yet been referenced specifically in any publications.
Publishing research using ab73536? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"