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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Overview
Product name
Anti-Ube2G1 antibody
See all Ube2G1 products (3) ...
Description
Mouse monoclonal to Ube2G1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-171 of Human Ube2G1
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1-5 µg/ml.
IF/ICC: Use at a concentration of 20 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.
Tissue specificity
Widely expressed, mainly in skeletal muscle.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Target information above from: UniProt accessionP62253
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E217K antibody
- UB2G1_HUMAN antibody
- UBC 7 antibody
see all
Database links
- Entrez Gene: 7326 Human
- Omim: 601569 Human
- SwissProt: P62253 Human
- Unigene: 727591 Human
Anti-Ube2G1 antibody images:
Immunofluorescence - Ube2G1 antibody (ab58256)
Ube2G1 antibody (ab58256) used in immunofluorescence at 20ug/ml on HeLa cells.
Western blot - Ube2G1 antibody (ab58256)
Ube2G1 antibody (ab58256) at 1ug/lane + HeLa cell lysate at 25ug/lane.
References for Anti-Ube2G1 antibody (ab58256)
ab58256 has not yet been referenced specifically in any publications.
Publishing research using ab58256? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"