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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Overview
Product name
Anti-Ube2H antibody
Description
Mouse monoclonal to Ube2H
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-184 of Human Ube2H
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1-5 µg/ml.
IF/ICC: Use at a concentration of 10 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Target information above from: UniProt accessionP62256
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2 20K antibody
- Ubc H2 antibody
- UBC8 antibody
see all
Database links
- Entrez Gene: 7328 Human
- Omim: 601082 Human
- SwissProt: P62256 Human
- Unigene: 643548 Human
- Unigene: 700585 Human
Anti-Ube2H antibody images:
Immunofluorescence - Ube2H antibody (ab58261)
Ube2H antibody (ab58261) used in immunofluorescence at 10ug/ml on HeLa cells.
Western blot - Ube2H antibody (ab58261)
Ube2H antibody (ab58261) at 1ug/lane + HeLa cell lysate at 25ug/lane.
References for Anti-Ube2H antibody (ab58261)
ab58261 has not yet been referenced specifically in any publications.
Publishing research using ab58261? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"