Anti-Ube2L3 antibody [EPR4368] (ab108936)

Overview

• Product nameAnti-Ube2L3 antibody [EPR4368]See all Ube2L3 primary antibodies ...
• Description
  Rabbit monoclonal [EPR4368] to Ube2L3
• Tested applicationsICC/IF, WB, IP, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A synthetic peptide corresponding to residues in Human Ube2L3

• Positive controlWB: HeLa, LNCaP, Jurkat and K562 cell lysates IHC-P: Human testis tissue IF: HeLa cells
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR4368
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Ubiquitin & Ubiquitin Like Modifiers
  • E2 Ubiquitin Conjugating Enzymes

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin E2s and E3s
  • Ubiquitin E2 Enzymes

Applications

Target

• FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
• Tissue specificityUbiquitous, with highest expression in testis.
• PathwayProtein modification; protein ubiquitination.
• Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
• Post-translational
  modificationsUbiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
• Cellular localizationNucleus. Cytoplasm.

Target information above from: UniProt accession P68036 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7332 Human
  • Entrez Gene: 100042355 Mouse
  • Entrez Gene: 22195 Mouse
  • Entrez Gene: 363836 Rat
  • Omim: 603721 Human
  • SwissProt: P68036 Human
  • SwissProt: P68037 Mouse
  • Unigene: 108104 Human

  • Unigene: 603229 Human
  • Unigene: 3074 Mouse
  • Unigene: 163186 Rat

  see all

• Alternative names
  E2 F1 antibodyL UBC antibodyL-UBC antibody

  UB2L3_HUMAN antibodyUBCE7 antibodyUbcH7 antibodyUbcM4 antibodyUbe2l3 antibodyUbiquitin carrier protein L3 antibodyUbiquitin conjugating enzyme E2 L3 antibodyUbiquitin protein ligase L3 antibodyUbiquitin-conjugating enzyme E2 L3 antibodyUbiquitin-conjugating enzyme E2-F1 antibodyUbiquitin-protein ligase L3 antibody

  see all

Anti-Ube2L3 antibody [EPR4368] images

• 

  Western blot - Ube2L3 antibody [EPR4368] (ab108936)
  All lanes : Anti-Ube2L3 antibody [EPR4368] (ab108936) at 1/1000 dilution
  
  Lane 1 : HeLa cell lysate
  Lane 2 : LNCaP cell lysate
  Lane 3 : Jurkat cell lysate
  Lane 4 : K562 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 18 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ube2L3 antibody [EPR4368] (ab108936)
  ab108936, at a 1/100 dilution, staining Ube2L3 in paraffin-embedded Human testis tissue
• 

  Immunocytochemistry/ Immunofluorescence - Ube2L3 antibody [EPR4368] (ab108936)
  ab108936, at a 1/100 dilution, staining Ube2L3 in HeLa cells