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Anti-Ube2L3 antibody (ab37913)

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Overview

Product name

Anti-Ube2L3 antibody
See all Ube2L3 products (9) ...

Description

Goat polyclonal to Ube2L3

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Chicken, Cow, Xenopus laevis, Zebrafish

Immunogen

Synthetic peptide: C-EFTKKYGEKRPVD, corresponding to C terminal amino acids 142/154 of Human Ube2L3

C-EFTKKYGEKRPVD

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Hela and Jurkat lysates

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> E2 Ubiquitin Conjugating Enzymes
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes

  • Western blot - Ube2L3 antibody (ab37913)Western blot - Ube2L3 antibody (ab37913) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab37913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: antibody detection limit dilution 1/128000.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.

Tissue specificity

Ubiquitous, with highest expression in testis.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Post-translational
modifications

Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.

Cellular localization

Nucleus. Cytoplasm.

Target information above from: UniProt accessionP68036 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • E2 F1 antibody
  • E2-F1 antibody
  • L UBC antibody
  • L-UBC antibody
  • UB2L3_HUMAN antibody
  • UBCE7 antibody
  • UbcH7 antibody
  • UbcM4 antibody
  • Ube2l3 antibody
  • Ubiquitin carrier protein L3 antibody
  • Ubiquitin conjugating enzyme E2 L3 antibody
  • Ubiquitin protein ligase L3 antibody
  • Ubiquitin-conjugating enzyme E2 L3 antibody
  • Ubiquitin-conjugating enzyme E2-F1 antibody
  • Ubiquitin-protein ligase L3 antibody
see all

Database links

see all

Anti-Ube2L3 antibody images:

  Western blot - Ube2L3 antibody (ab37913)

Western blot - Ube2L3 antibody (ab37913)

Anti-Ube2L3 antibody (ab37913) at 0.5 µg/ml + Hela lysate (RIPA buffer) at 35 µg
developed using the ECL technique

Predicted band size : 18 kDa
Observed band size : 18 kDa


Primary incubated for 1 hour.

References for Anti-Ube2L3 antibody (ab37913)

This product has been referenced in:

  • Reyes-Hernández OD  et al. Ube2l3 gene expression is modulated by activation of the aryl hydrocarbon receptor: implications for p53 ubiquitination. Biochem Pharmacol 80:932-40 (2010). WB; Mouse.Read more (PubMed: 20478272) »

See 1 publication for this product

Publishing research using ab37913? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"