Recombinant Anti-Ube2L6 antibody [EPR4267(2)] (ab109086)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4267(2)] to Ube2L6
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Ube2L6 antibody [EPR4267(2)]
See all Ube2L6 primary antibodies -
Description
Rabbit monoclonal [EPR4267(2)] to Ube2L6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal thymus, Human lymph node, Daudi, and MOLT4 lysates; Human kidney and uterus tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4267(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Target
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Function
Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. -
Tissue specificity
Present in natural killer cells (at protein level). -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. -
Post-translational
modificationsISGylated. - Information by UniProt
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Database links
- Entrez Gene: 9246 Human
- Omim: 603890 Human
- SwissProt: O14933 Human
- Unigene: 425777 Human
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Alternative names
- MGC40331 antibody
- Retinoic acid induced gene B protein antibody
- Retinoic acid-induced gene B protein antibody
see all
Images
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All lanes : Anti-Ube2L6 antibody [EPR4267(2)] (ab109086) at 1/1000 dilution
Lane 1 : Human fetal thymus tissue lysate
Lane 2 : Human lymph node tissue lysate
Lane 3 : Daudi cell lysate
Lane 4 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 18 kDa -
ab109086 at 1/250 dilution staining Ube2L6 in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab109086 at 1/250 dilution staining Ube2L6 in Human uterus by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab109086 has been referenced in 4 publications.
- Oki N et al. Curcumin partly prevents ISG15 activation via ubiquitin-activating enzyme E1-like protein and decreases ISGylation. Biochem Biophys Res Commun 625:94-101 (2022). PubMed: 35952613
- Ke M Systematic Analysis of Molecular Subtypes Based on the Expression Profile of Immune-Related Genes in Pancreatic Cancer. Oxid Med Cell Longev 2022:3124122 (2022). PubMed: 36567857
- Zhang Q et al. UHRF1 epigenetically down-regulates UbcH8 to inhibit apoptosis in cervical cancer cells. Cell Cycle 17:300-308 (2018). PubMed: 29157076
- Sooryanarain H et al. ISG15 Modulates Type I Interferon Signaling and the Antiviral Response during Hepatitis E Virus Replication. J Virol 91:N/A (2017). PubMed: 28724761