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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Overview
Product name
Anti-Ube2L6 antibody - N-terminal
See all Ube2L6 products (5) ...
Description
Rabbit polyclonal to Ube2L6 - N-terminal
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the N-terminal region of human Ube2L6 conjugated to KLH.
Positive control
Human hepatocarcinoma tissue. HL-60 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab71800 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Ubiquitin like Modifiers
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> E2 Ubiquitin Conjugating Enzymes
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 18 kDa. (predicted molecular weight: 18 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53.
Tissue specificity
Present in natural killer cells (at protein level).
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Post-translational
modifications
ISGylated.
Target information above from: UniProt accessionO14933
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC40331 antibody
- Retinoic acid induced gene B protein antibody
- Retinoic acid-induced gene B protein antibody
see all
Database links
- Entrez Gene: 9246 Human
- Omim: 603890 Human
- SwissProt: O14933 Human
- Unigene: 425777 Human
Anti-Ube2L6 antibody - N-terminal images:
Western blot - Ube2L6 antibody - N-terminal (ab71800)
Anti-Ube2L6 antibody - N-terminal (ab71800) at 1/100 dilution + HL-60 cell lysate at 12.5 µg
Predicted band size : 18 kDa
Observed band size : 18 kDa
Additional bands at : 30 kDa,60 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ube2L6 antibody - N-terminal (ab71800)
ab71800, at 1/50 dilution, staining Ube2L6 in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
References for Anti-Ube2L6 antibody - N-terminal (ab71800)
This product has been referenced in:
- Makki MSet al. TSA downregulates Wilms tumor gene 1 (Wt1) expression at multiple levels. Nucleic Acids Res 36:4067-78 (2008). Human.Read more (PubMed: 18535006) »
See 1 publication for this product
Publishing research using ab71800? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"