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Overview
Product name
Anti-Ube2M antibody
See all UBE2M products (6) ...
Description
Rabbit polyclonal to Ube2M
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
KLH-conjugated synthetic peptide (10-30 amino acids in length), selected from the C terminal of last 50 amino acids of Human Ube2M .
Positive control
Human breast carcinoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Ubiquitin like Modifiers
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/50 - 1/100.
Target
Function
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
Pathway
Protein modification; protein neddylation.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
Domain
Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
Target information above from: UniProt accessionP61081
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hUbc12 antibody
- NEDD8 carrier protein antibody
- NEDD8 conjugating enzyme Ubc12 antibody
see all
Database links
- Entrez Gene: 9040 Human
- Omim: 603173 Human
- SwissProt: P61081 Human
- Unigene: 406068 Human
Anti-Ube2M antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ube2M antibody (ab38870)
ab38870, at 1/50 dilution, staining Ube2M in Human breast carcinoma, by Immunohistochemistry, Formalin fixed and Paraffin embedded tissue. ab38870 was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
References for Anti-Ube2M antibody (ab38870)
ab38870 has not yet been referenced specifically in any publications.
Publishing research using ab38870? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"