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Overview
Product name
Anti-Ube2M antibody
See all UBE2M products (6) ...
Description
Mouse monoclonal to Ube2M
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-184 of Human Ube2M
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Ubiquitin like Modifiers
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Western blot - Ube2M antibody (ab56383)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
Pathway
Protein modification; protein neddylation.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
Domain
Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
Target information above from: UniProt accessionP61081
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hUbc12 antibody
- NEDD8 carrier protein antibody
- NEDD8 conjugating enzyme Ubc12 antibody
see all
Database links
- Entrez Gene: 9040 Human
- Omim: 603173 Human
- SwissProt: P61081 Human
- Unigene: 406068 Human
Anti-Ube2M antibody images:
Western blot - Ube2M antibody (ab56383)
Ube2M antibody (ab56383) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
References for Anti-Ube2M antibody (ab56383)
ab56383 has not yet been referenced specifically in any publications.
Publishing research using ab56383? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"