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Overview
Product name
Anti-Ube2N / Ubc13 antibody [4E11]
See all Ube2N / Ubc13 products (8) ...
Description
Mouse monoclonal [4E11] to Ube2N / Ubc13
Specificity
This antibody is specific for the ~21 kDa Ubc13 protein.
Tested applications
WB, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant full length protein (Human)
Positive control
Human HepG2 cell lysates, mouse NIH 3T3 cells, and rat Rat2 fibroblasts.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Highly purified from mouse ascites
Clonality
Monoclonal
Clone number
4E11
Isotype
IgG1
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> E2 Ubiquitin Conjugating Enzymes
Immunology >> Innate Immunity >> TLR Signaling
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 3 µg/ml.Predicted molecular weight: 17 kDa.
ELISA: Use a concentration of 0.1 - 1.0 µg/ml.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Target
Function
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Post-translational
modifications
Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
Cellular localization
Nucleus. Cytoplasm.
Target information above from: UniProt accessionP61088
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Bendless like ubiquitin conjugating enzyme antibody
- Bendless-like ubiquitin-conjugating enzyme antibody
- BLU antibody
see all
Database links
- Entrez Gene: 7334 Human
- Entrez Gene: 93765 Mouse
- Entrez Gene: 116725 Rat
- Omim: 603679 Human
- SwissProt: P61088 Human
- SwissProt: P61089 Mouse
- SwissProt: Q9EQX9 Rat
- Unigene: 524630 Human
see all
References for Anti-Ube2N / Ubc13 antibody [4E11] (ab38795)
This product has been referenced in:
- Oveland Eet al. Ligand-induced Flt3-downregulation modulates cell death associated proteins and enhances chemosensitivity to idarubicin in THP-1 acute myeloid leukemia cells. Leuk Res 33:276-87 (2009). WB; Human.Read more (PubMed: 18691757) »
- Andersen PLet al. Distinct regulation of Ubc13 functions by the two ubiquitin-conjugating enzyme variants Mms2 and Uev1A. J Cell Biol 170:745-55 (2005).Read more (PubMed: 16129784) »
See all 2 publications for this product
Publishing research using ab38795? Please let us know so that we can cite the reference in this datasheet
![Ube2N antibody [4E11] for ICC/IF in Human (38795)](/ps/Reviews/Images/ab8149_38056.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"