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Overview
Product name
Anti-Ube2N / Ubc13 antibody
See all Ube2N / Ubc13 products (8) ...
Description
Rabbit polyclonal to Ube2N / Ubc13
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Xenopus laevis, Zebrafish
Immunogen
Mixture of synthetic peptides containing amino acids 2-19 (AGLPRRIIKETQRLLAEP) and 131-148 (TNEAQAIETARAWTRLYA) of Human UBE2N.
TNEAQAIETA RAWTRLYA
Positive control
Human heart lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> E2 Ubiquitin Conjugating Enzymes
Immunology >> Innate Immunity >> TLR Signaling
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Western blot - Ube2N antibody (ab13663)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/mlDetects a band of approximately 17 kDa (predicted molecular weight: 18.24 kDa).
Target
Function
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Post-translational
modifications
Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
Cellular localization
Nucleus. Cytoplasm.
Target information above from: UniProt accessionP61088
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bendless like ubiquitin conjugating enzyme antibody
- Bendless-like ubiquitin-conjugating enzyme antibody
- BLU antibody
see all
Database links
- Entrez Gene: 7334 Human
- Entrez Gene: 93765 Mouse
- Entrez Gene: 116725 Rat
- Entrez Gene: 380524 Xenopus laevis
- Entrez Gene: 406807 Zebrafish
- Omim: 603679 Human
- SwissProt: P61088 Human
- SwissProt: P61089 Mouse
see all
Anti-Ube2N / Ubc13 antibody images:
Western blot - Ube2N antibody (ab13663)
Predicted band size : 18.24 kDa
Detection of Ube2N in human heart lysate by Western blot (ECL) with ab13663 at 2 µg/ml.
References for Anti-Ube2N / Ubc13 antibody (ab13663)
ab13663 has not yet been referenced specifically in any publications.
Publishing research using ab13663? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"