Ube2N antibody (ab25885)

Product Name 

Ube2N antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Ube2N

Immunogen 

Synthetic peptide, corresponding to 15 amino acids near the C terminus of Human Ube2N.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at a concentration of 2 µg/ml.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 18 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human small intestine or HepG2 cell lysate.

Cellular localization 

Nuclear

Research areas 

Immunology >> Innate Immunity >> TLR Signaling
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation

Relevance 

Ube2N is a member of the E2 ubiquitin conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short lived proteins for degradation. Ube2N (ubiquitin conjugating enzyme E2N) is a novel human ubiquitin conjugating enzyme with an ability to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme E1 dependent manner. The corresponding transcripts are highly expressed in heart, skeletal muscle, and testis. Ube2N protein may be involved in protein degradation mainly in the muscles and testis. The Ube2V2/Ube2N heterodimer catalyzes the synthesis of non-canonical poly ubiquitin chains that are linked through Lys63. This type of poly ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error free DNA repair pathway and contributes to the survival of cells after DNA damage.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Storage buffer 

Preservative: 0.02% Sodium Azide.
Constituents: PBS.

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody was purified by Ion exchange chromatography.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

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