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Western blot - Ube2N antibody (ab25885)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ube2N antibody (ab25885)
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Product Name
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Ube2N antibody
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See all Ube2N antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Ube2N
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Immunogen
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Synthetic peptide, corresponding to 15 amino acids near the C terminus of Human Ube2N.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 2 µg/ml.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 18 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human small intestine or HepG2 cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Immunology >> Innate Immunity >> TLR Signaling
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Relevance
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Ube2N is a member of the E2 ubiquitin conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short lived proteins for degradation. Ube2N (ubiquitin conjugating enzyme E2N) is a novel human ubiquitin conjugating enzyme with an ability to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme E1 dependent manner. The corresponding transcripts are highly expressed in heart, skeletal muscle, and testis. Ube2N protein may be involved in protein degradation mainly in the muscles and testis. The Ube2V2/Ube2N heterodimer catalyzes the synthesis of non-canonical poly ubiquitin chains that are linked through Lys63. This type of poly ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error free DNA repair pathway and contributes to the survival of cells after DNA damage.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Storage buffer
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Preservative: 0.02% Sodium Azide.
Constituents: PBS. Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody was purified by Ion exchange chromatography.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Ube2N antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Ube2N antibody images, references, products related to ab25885 and other tools.
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Ube2N antibody images:
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 Western blot - Ube2N antibody (ab25885)
All lanes : Ube2N antibody (ab25885) (analysis
in human
small intestine cell lysates 15ug/lane
)
Lane 1 : 0.5 ug/ml of antibody.
Lane 2 : 1 ug/ml of antibody.
Lane 3 : 2 ug/ml of antibody.
Predicted band size : 18 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ube2N antibody (ab25885)
ab25885 at 2µg/ml staining UBe2N in mouse thymus tissue by IHC
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Search PubMed (MEDLINE) for references to Ube2N
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Ube2N antibody - more information
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Customer reviews (feedback) regarding Ube2N antibody |
Customer FAQs regarding Ube2N antibody |
Protocols for Ube2N antibody |
Price and availability of products related to Ube2N antibody |
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Ube2N antibody - related products:
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Ube2N antibody - other tools:
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Contact Abcam with a Technical Enquiry about Ube2N antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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