Overview

  • Product name
    Anti-Ubiquilin antibody
    See all Ubiquilin primary antibodies
  • Description
    Rabbit polyclonal to Ubiquilin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Hamster, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Ubiquilin aa 2-18.
    Sequence:

    AESAESGGPPGAQDSAA

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • CHO cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab3341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.

By Western blot, this antibody detects an ~63 kDa protein representing Ubiquilin from CHO cell extract.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml. PubMed: 19112176

Target

  • Function
    Links CD47 to the cytoskeleton. Promotes the surface expression of GABA-A receptors (By similarity). Promotes the accumulation of uncleaved PSEN1 and PSEN2 by stimulating their biosynthesis. Has no effect on PSEN1 and PSEN2 degradation.
  • Tissue specificity
    Ubiquitous. Highly expressed throughout the brain; detected in neurons and in neuropathological lesions, such as neurofibrillary tangles and Lewy bodies. Highly expressed in heart, placenta, pancreas, lung, liver, skeletal muscle and kidney.
  • Sequence similarities
    Contains 1 UBA domain.
    Contains 1 ubiquitin-like domain.
  • Domain
    Binds PSEN1 and PSEN2 via its UBA domain.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • DA41 antibody
    • DSK2 antibody
    • FLJ90054 antibody
    • hPLIC-1 antibody
    • hPLIC1 antibody
    • PLIC-1 antibody
    • PLIC1 antibody
    • Protein linking IAP with cytoskeleton 1 antibody
    • Ubiquilin-1 antibody
    • Ubiquilin1 antibody
    • UBQL1_HUMAN antibody
    • UBQLN1 antibody
    • UBQN antibody
    • XDRP1 antibody
    see all

Images

  • Western blot - Anti-Ubiquilin antibody (ab3341)
    Western blot - Anti-Ubiquilin antibody (ab3341)

    Western blot detection of Ubiquilin on CHO cell extract using ab3341.

References

This product has been referenced in:
  • Adegoke OO  et al. Overexpression of Ubiquilin-1 Alleviates Alzheimer's Disease-Caused Cognitive and Motor Deficits and Reduces Amyloid-ß Accumulation in Mice. J Alzheimers Dis 59:575-590 (2017). WB . Read more (PubMed: 28598849) »
  • Adusei DC  et al. Early developmental alterations in GABAergic protein expression in fragile X knockout mice. Neuropharmacology 59:167-71 (2010). WB ; Mouse . Read more (PubMed: 20470805) »

See all 3 Publications for this product

Publishing research using ab3341? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

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Western blot

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (U2OS)
Specification
U2OS
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Username

Dr. Randal Tibbetts

Verified customer

Submitted May 23 2013

used same Ab for 2 years
lot 925125 worked
lot GR27748-2 did not work
mouse cell lysate
lots of bands: 66 kDa and many more bands
same samples
Ab: 1/1000 o.n 4 degree
block: 5% milk
2nd: OK

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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