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Product name
Anti-Ubiquitin +1 antibody [40B3]
Description
Mouse monoclonal [40B3] to Ubiquitin +1
Tested applications
ELISA, IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human) conjugated to KLH
Positive control
Bosc23 cell lysate transfected with GFP Ub +1.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
40B3
Isotype
IgG1
Light chain type
kappa
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Proteasome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
IP
WB
Application notes
ELISA: Use at an assay dependent dilution.
IP: Use at a concentration of 1 - 2 µg/ml cell lysate.
WB: 1/500 - 1/1000. Predicted molecular weight: 9 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Ubiquitin +1 (Ub+1) is a novel mutant form of ubiquitin that can be produced through a process known as molecular frameshift. Ub+1 can be polyubiquitinated to produce aberrant polyubiquitin chains that inhibit the 26S proteasome. Especially Ub+1 is accumulated in aggregates containing amyloid beta and phosphorylated tau. Elevated expression of Ub+1 mRNA and protein has been observed in the brains of patients with Alzheimer's disease. Also Ub+1 acts as an aggravating factor in polyglutamine induced neurodegeneration.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- UBB antibody
- Ubiquitin antibody
- Ubiquitin B antibody
Database links
- Entrez Gene: 7314 Human
- Omim: 191339 Human
- SwissProt: P62988 Human
- Unigene: 356190 Human
References for Anti-Ubiquitin +1 antibody [40B3] (ab24259)
ab24259 has not yet been referenced specifically in any publications.
Publishing research using ab24259? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"