Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
If your product does not perform as described on this datasheet, we will refund or replace your product...
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I have purchased 2 vials of ab7254 recently. They are both the same lot number. The first one worked well but the second one is detecting very little or no ubiquitin. I have used an antibody against the ubiquitinated protein to prove it is there and I see ub forms of this protein with that antibody. |
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ANSWER: |
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab7254. |
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I used to anti-ubiquitin antibodies with the same free floating sections with antigen pH 6 retrieval buffer using mouse brain. One antibody worked great (ab7254) and another ab7780 didn't stain at all. |
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ANSWER: |
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab7254. |
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I’m in AB4587 and don’t see a link to MSDS. I see the Data sheet link to the right, but no link to msds. Could you look at this page and tell me where it is? http://www.abcam.com/Alzheimer-brain-human-temporal-lobe-tissue-slides-ab4587.html |
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ANSWER: |
Thanks for your email. ab4587 does not have any required MSDS information, so a link will not be present on this datasheet. For example, ab7254 does have a link to MSDS information. I hope this helps. Please contact me again if you have any further questions. |
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I’m looking for MSDS for the items below. B-Amyloid Control Slides (5 Slides/PK) ABCAM AB4587 BCL2 Antibody ABCAM AB32124 BETA Amyloid ABCAM BLIMP-1 ABCAM AB81961 FOXP3 ANTIBODY (236A/E7) ABCAM WIHC031 GCET1 ABCAM AB68889 GPNMB Antibody (Monoclonal) (100ug) ABCAM AB56584 Human Milk Fat Globule 1 Antibody (500 uL) ABCAM AB17787 IMP-3 antibody ABCAM AB82044 Mesothelioma Positive Controls ABCAM AB4437 Oct1 Antibody (500uL) ABCAM AB15112 SMOOTHELIN ANTIBODY ABCAM TFE-3 ABCAM AB70008 Ubiquitin ABCAM AB7254 |
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ANSWER: |
Thanks for your enquiry. If required, MSDS information is located via one or more 'MSDS' links near the top left corner of the online datasheet. This link is just below the 'Datasheet PDF' link present on all datasheets. I hope this is helpful. Please contact me again if you have any further questions. |
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Testing discounts for ab69845 and ab7254 in IHC-FoFr |
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ANSWER: |
DISCOUNT CODE: *******Expiration date:****** I am very pleased to hear you would like to accept our offer and test ab7254 in IHC-FoFr. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-FoFr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Human normal placenta. Staining is localised in the cytoplasm and in the nuclei. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus, at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab7254 staining Ubiquitin in murine myocytes by Immunocytochemistry/ Immunofluorescence.Isolated myocytes were treated with 10µM FCCP for 1 hour, or untreated as the control. Cells were then fixed in formaldehyde, permeabilized using 1% Triton X-100, then blocked with 10% horse serum for 1 hour at room temperature, incubated with ab7254 at a 1/200 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal, used at a 1/500 dilution.Results show that with FCCP treatment, Ubiquitin is recruited onto mitochondria.
Image courtesy of an anonymous Abreview.
HeLa cells were co-transfected with a plasmid expressing a target protein together with Ubi expressing vector for 24 hours and either left untreated (Contr) or were treated with 10 µM MG-132 for 6 hours (+MG132). Then the protein of interest was pulled down using Flag agarose beads and and probed with ab7254 at a 1/2000 dilution. The secondary used was an Alexa-Fluor 680 conjugated goat anti-mouse polyclonal used at a 1/10000 dilution. The protein is known to be degraded through proteasome.
Image courtesy of an anonymous Abreview.
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