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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Overview
Product name
Anti-Ubiquitin antibody
See all Ubiquitin products (31) ...
Description
Rabbit polyclonal to Ubiquitin
Specificity
Ab14372 recognises ubiquitin.
Tested applications
WB, ELISA, ICC/IF, Flow Cytmore details
Cross reactivity
Reacts with
Human, Saccharomyces cerevisiae
Immunogen
Ubiquitin coupled to rabbit IgG.
Positive control
Most yeast cell lysates can be used as a positive control without induction or stimulation.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This antibody was purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitation arc against anti-Rabbit Serum.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 8.5 kDa (predicted molecular weight: 8.5 kDa).
ELISA
ELISA: 1/2000 - 1/10000.ELISA: 1/2000 - 1/10000.
ICC/IF: 1/100(See Abreview.)
Flow Cyt: 1/200((see Abreview).)
Target
Relevance
Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.
Cellular localization
Cell Membrane, Cytoplasmic and Nuclear
Alternative names
- FLJ25987 antibody
- MGC8385 antibody
- Polyubiquitin B antibody
see all
Database links
- Entrez Gene: 7314 Human
- Omim: 191339 Human
- SwissProt: P0CG47 Human
- Unigene: 356190 Human
Anti-Ubiquitin antibody images:
Western blot - Ubiquitin antibody (ab14372)
Predicted band size : 8.5 kDa
ab14372 was tested against E.coli lysates expressing a ubiquitin GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other. Therefore it is important to know the degree of reactivity of each antibody against each UBL.
Panle A: shows total protein staining using Ponceau. Panel B: shows specific reaction with ubiquitin using a 1:1,000 and 1;2,000 dilution of ab14372 followed by an appropriate secondary antibody. The data indicate that ab14372 is highly specific and does not cross react with other UBLs.
Western blot - Ubiquitin antibody (ab14372)
Predicted band size : 8.5 kDa
ab14372 at dilution 1:200 (left lane) and 1:1000 (right lane) was tested by immunoblot against total cell extract from yeast. The blots shows strong reactivity of ab14372 with ubiquitinated proteins. Ab14372 was used at 1/500 dilution and incubated overnight at 4°C in 5% non-fat dry milk in TTBS.
Immunocytochemistry/ Immunofluorescence - Ubiquitin antibody (ab14372)
Central Panel: ab14372 staining Ubiquitin in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 2% BSA for 1 hour. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 568-conjugated Goat polyclonal to rabbit IgG, dilution 1/250, was used as secondary antibody.
Left-hand panel: Nuclei counterstained with DAPI (blue).
Right-hand panel: Overlay
This image is courtesy of an anonymous Abreview
Flow Cytometry - Ubiquitin antibody (ab14372)
ab14372 staining Ubiquitin in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.
Image courtesy of Dr Mahesh Shivananjappa by Abreview.
References for Anti-Ubiquitin antibody (ab14372)
This product has been referenced in:
- Zimmermann Cet al. A chemical-genetic screen to unravel the genetic network of CDC28/CDK1 links ubiquitin and Rad6-Bre1 to cell cycle progression. Proc Natl Acad Sci U S A 108:18748-53 (2011).Read more (PubMed: 22042866) »
- den Dulk Bet al. The NER protein Rad33 shows functional homology to human Centrin2 and is involved in modification of Rad4. DNA Repair (Amst) 7:858-68 (2008).Read more (PubMed: 18387345) »
See all 2 publications for this product
Publishing research using ab14372? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"