Anti-Ubiquitin antibody (ab27268)

Overview

• Product nameAnti-Ubiquitin antibodySee all Ubiquitin primary antibodies ...
• Description
  Rabbit polyclonal to Ubiquitin
• SpecificityThis antibody recognises ubiquitin.
• Tested applicationsICC/IF, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length human ubiquitin protein.

• Positive controlHuman Alzheimer’s brain for Immunohistochemistry.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 1% BSA, 10mM PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis antibody is a affinity purified.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Ubiquitylation

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Tangles & Tau

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin

Applications

Target

• RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
• Cellular localizationCell Membrane, Cytoplasmic and Nuclear
• Database links
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human

  • Omim: 191339 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human

  see all

• Alternative names
  FLJ25987 antibodyMGC8385 antibodyPolyubiquitin B antibody

  RPS 27A antibodyRPS27A antibodyUBA 52 antibodyUBA 80 antibodyUBA52 antibodyUBA80 antibodyUBB antibodyUBB_HUMAN antibodyUBC antibodyUBCEP 1 antibodyUBCEP 2 antibodyUBCEP1 antibodyUBCEP2 antibodyUbiquitin antibodyubiquitin B antibody

  see all

Anti-Ubiquitin antibody images

• 

  Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Ubiquitin antibody (ab27268)
  Ab27268, prediluted, staining Ubiquitin in paraffin embedded, formalin fixed human Alzheimer’s brain tissue by Immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence-Ubiquitin antibody(ab27268)
  ICC/IF image of ab27268 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27268, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.