Anti-Ubiquitin antibody (ab7780)

Immunohistochemistry (PFA perfusion fixed frozen sections) - Ubiquitin antibody (ab7780)

ab7780 at a dilution of 1/1000, staining Ubiquitin in neurons (Alexa 488 secondary at 1/2000) on 30µm coronal rat brain tissue sections in free floating IHC (see protocol link for detailed description). Image shows neuronal staining observed with [A] 20x objective and [B] 40x objective.

NB: No labeling observed following omission of primary antibody.

Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ubiquitin antibody (ab7780)

ab7780 (1/100) staining Ubiquitin in paraffin-embedded brain sections of a patient with Alzheimer's disease.

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody (ab7780)

ICC/IF image of ab7780 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7780, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody (ab7780)

ab7780 staining Ubiquitin in Panc-1 cells by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in formaldehyde, blocked with 1% serum for 1 hour at 22°C and then incubated with ab7780 at a 1/2000 dilution for 1 hour at 22°C. The secondary was used at a 1/200 dilution.

Green - Golgi residental proteins C2GnT-M.
Red -Ubiquitin.
Blue - nucleus staining by DAPI.

Image courtesy of Armen Petrosyan by Abreview.