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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Overview
Product name
Anti-Ubiquitin antibody
See all Ubiquitin products (31) ...
Description
Rabbit polyclonal to Ubiquitin
Specificity
This antibody reacts with ubiquitin, a polypeptide w/ Mr. of approx. 8.5kD. Reacts with physiologically occurring conjugates of ubiquitin and intracellular proteins. Specifically recognizes ubiquitinated cytoplasmic inclusion bodies.
Tested applications
ICC/IF, ELISA, IHC-FoFr, IHC-P, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, African Green Monkey
Predicted to work with
Horse, Cow
Immunogen
Recombinant full length protein (Human)
Positive control
Pancreas and Alzheimer’s brain
General notes
This antibody stains the periphery of senile plaques and of neurofibrillary tangles in Alzheimer's disease brain, the Lewy bodies in Parkinson's disease brain, and the Mallory bodies in alcoholic liver disease.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: Sodium Azide
Constituents: BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (1/500 - 1/1000.)
ELISA: Use at an assay dependent dilution.
IHC-FoFr: 1/50 - 1/100.
IHC-P: 1/50 - 1/100.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.( Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
WB: Use at an assay dependent dilution. Predicted molecular weight: 8.5 kDa.(Used at a dilution of 1/2000 B16F10 murine melanoma cell line (see Abreview). Predicted molecular weight: 8.5 kDa. )
IP: Use at an assay dependent concentration.
Target
Relevance
Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.
Cellular localization
Cell Membrane, Cytoplasmic and Nuclear
Alternative names
- FLJ25987 antibody
- MGC8385 antibody
- Polyubiquitin B antibody
see all
Database links
- Entrez Gene: 281370 Cow
- Entrez Gene: 100049794 Horse
- Entrez Gene: 7314 Human
- Entrez Gene: 22187 Mouse
- Entrez Gene: 192255 Rat
- Omim: 191339 Human
- SwissProt: P0CG53 Cow
- SwissProt: Q8MKD1 Horse
see all
Anti-Ubiquitin antibody images:
Immunohistochemistry (PFA perfusion fixed frozen sections) - Ubiquitin antibody (ab7780)
ab7780 at a dilution of 1/1000, staining Ubiquitin in neurons (Alexa 488 secondary at 1/2000) on 30
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ubiquitin antibody (ab7780)
ab7780 (1/100) staining Ubiquitin in paraffin-embedded brain sections of a patient with Alzheimer's disease.
Immunocytochemistry/ Immunofluorescence-Ubiquitin antibody(ab7780)
ICC/IF image of ab7780 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7780, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
References for Anti-Ubiquitin antibody (ab7780)
This product has been referenced in:
- Sánchez-Quiles Vet al. Prohibitin deficiency blocks proliferation and induces apoptosis in human hepatoma cells: molecular mechanisms and functional implications. Proteomics 10:1609-20 (2010). WB; Human.Read more (PubMed: 20186755) »
- Darwanto Aet al. A modified "cross-talk" between histone H2B Lys-120 ubiquitination and H3 Lys-79 methylation. J Biol Chem 285:21868-76 (2010). WB; Human.Read more (PubMed: 20442396) »
See all 6 publications for this product
Publishing research using ab7780? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"