Overview

  • Product name
    Universal HIER antigen retrieval reagent (10X)
  • Product overview

    Abcam’s Universal HIER antigen retrieval reagent (10X) is a heat-induced epitope retrieval (HIER) solution that is compatible with virtually all antibodies and eliminates the need for multiple buffers, including citrate buffer, EDTA or high pH Tris buffers. Antibody titers are doubled or tripled when compared to citrate buffer, pH 6.0. Universal HIER antigen retrieval reagent (10X) incorporates a color-coded high temperature pH indicator solution, allowing the end-user to check that the solution is at the correct dilution and pH by visual inspection. This product is specially formulated for superior pH stability at high temperatures in order to help prevent the possibility of losing pH-sensitive antigens. Universal HIER antigen retrieval reagent (10X) is non-toxic, non-flammable, odorless and sodium azide- and thimerosal-free.


     


    Ab208572 is the recommended HIER antigen retrieval reagent for
    PD-L1 RabMAb (clone 28-8, Ab205921).

  • Notes

    The Universal HIER antigen retrieval reagent is a buffered solution that is intended for laboratory use in heat induced antigen retrieval of formalin-fixed paraffin-embedded (FFPE) tissues for immunohistochemistry (IHC) procedures. The clinical interpretation of any staining or its absence should be complimented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

  • Tested applications
    Suitable for: IHCmore details

Properties

  • Storage instructions
    Store at room temperature. Please refer to protocols.
  • Components 100 ml
    Universal HIER antigen retrieval reagent 1 x 100ml

Applications

Our Abpromise guarantee covers the use of ab208572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use at an assay dependent concentration.

Images

  • Human tonsil stained with PD-L1 RabMAb antibody (clone 28-8, Ab205921) using Universal HIER antigen retrieval reagent (ab208572).

     

  • Human tonsil stained with rabbit monoclonal [28-8] to PD-L1 using Universal HIER antigen retrieval reagent (ab208572).

  • Human melanoma stained with rabbit monoclonal [28-8] to PD-L1 using Universal HIER antigen retrieval reagent (ab208572).

Protocols

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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