Overview
Product nameUnrip protein (Tagged-His Tag)See all Unrip proteins and peptides ...
Protein descriptionRecombinant fragment, corresponding to amino acids 119-350 of Human Unrip with an N terminal His tag; Predicted MWt: 27 kDa; UniProt ID: Q9Y3F4.
Expression hostE. coli
Properties
Purification notesPurity level >85%
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography.
In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag.
In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight.
This may be as a result of unequally distributed charges in the amino acid sequence.
Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
FormLyophilised:Reconstitute with 54 µl aqua dest.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Concentration information loading...
Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
Sequence notesGQDKLLRIYDLNKPEAEPKEISGHTSGIKKALWCSEDKQI LSADDKTV
RLWDHATMTEVKSLNFNMSVSSMEYIPEGE ILVITYGRSIAFHSAVSL
DPIKSFEAPATINSASLHPE KEFLVAGGEDFKLYKYDYNSGEELESYK
GHFGPIHCVR FSPDGELYASGSEDGTLRLWQTVVGKTYGLWKCVLPEE DSGELAKPKIGFPETTEEELEEIASENSDCIFPSAPDVKA
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab91788
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
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| SDS-PAGE |
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Application notesMS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
MAP activator with WD repeatsMAWDPTWD
serine/threonine kinase receptor associated proteinSTRAPunr interacting proteinUNR-interacting proteinUNRIPWD 40 repeat protein PT WD
see all
RelevanceUnrip is part of the SMN complex that plays a role in snRNP assembly in the cytoplasm and pre mRNA splicing in the nucleus. Unrip interacts directly with Gemin 6 and Gemin 7 in the SMN complex. It is associated with the complex in the cytoplasm but not in the nucleus and thus is thought to play a role in it's subcellular localisation.
It is a ubiquitously expressed protein that acts as an inhibitor of TGF-beta signaling and an important regulator of cell proliferation. Stable expression of STRAP results in activation of the mitogen-activated protein kinase/extracellular signal-regulated kinase pathway and in down-regulation of the cyclin-dependent kinase inhibitor p21 (Cip1), which results in retinoblastoma protein hyperphosphorylation. Upregulation of STRAP might play a role in tumor development as an early event for colorectal cancers. It is the first component of the U snRNP assembly machinery that associates with SMN complex in a compartment-specific way and plays a crucial role in the intracellular distribution of SMN1.
Cellular localizationCytoplasm. Nucleus. Note: Localized predominantly in the cytoplasm but also found in the nucleus.
Unrip protein (Tagged-His Tag) images
References for Unrip protein (Tagged-His Tag) (ab91788)
ab91788
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - Unrip protein (Tagged-His Tag) (ab91788)The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.