Uric Acid Assay Kit (ab65344)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Overview
-
Product name
Uric Acid Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Assay time
0h 40m -
Product overview
Uric Acid Assay Kit (ab65344) provides a convenient means for detecting uric acid in biological samples such as serum and urine. Pretreatment of samples is not required.
Uric acid level can be measured using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ= 570 nm) methods.
Uric acid assay protocol summary:
- add reaction mix to samples and standards
- incubate for 30 min
- analyze on microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K608 Uric Acid Colorimetric/Fluorometric Assay Kit. K608-100 is the same size as the 100 test size of ab65344.
Uric acid in serum is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration. However, human often lacks the necessary enzyme called urate oxidase (Uricase), and therefore abnormal uric acid may be accumulated in blood. Recent evidences show the close association between serum urate level and cardiovascular morbidity and mortality, especially among persons at high cardiovascular risk, including those with hypertension, diabetes and congestion heart failure.
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer II 1 x 25ml OxiRed Probe 1 x 0.2ml Uric Acid Enzyme Mix 1 vial Uric Acid Standard 1 x 1ml -
Research areas
-
Relevance
Uric acid is a heterocyclic purine derivate that is the final oxidation product of purine metabolism. It is produced by xantine oxidase, which oxidizes oxypurines such as xanthine into uric acid. In most mammals, except humans and higher primates, the enzyme uricase further oxidizes uric acid to allantoin. In humans, however, uric acid is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration and excreted in urine. Humans procude only small quantities of uric acid and therefore abnormal uric acid may be accumulated in blood leading to a type of arthritis known as gout. -
Alternative names
- Urate
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (25)
ab65344 has been referenced in 25 publications.
- Li C et al. Mechanisms of innate and adaptive immunity to the Pfizer-BioNTech BNT162b2 vaccine. Nat Immunol 23:543-555 (2022). PubMed: 35288714
- Abdulhafiz F et al. Acute oral toxicity assessment and anti-hyperuricemic activity of Alocasia longiloba extracts on Sprague-Dawley rats. Saudi J Biol Sci 29:3184-3193 (2022). PubMed: 35844413
- Potue P et al. Effects of Thai native chicken breast meat consumption on serum uric acid level, biochemical parameters, and antioxidant activities in rats. Sci Rep 12:14056 (2022). PubMed: 35982129
- Mohamed ME et al. The Protective Effect of Anethole against Renal Ischemia/Reperfusion: The Role of the TLR2,4/MYD88/NF?B Pathway. Antioxidants (Basel) 11:N/A (2022). PubMed: 35326185
- Kim BJ et al. Endurance Exercise Training Prevents Elevation of Soluble ST2 in Mice with Doxorubicin-Induced Myocardial Injury. Int J Heart Fail 3:59-68 (2021). PubMed: 36263109