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Anti-Urokinase antibody (ab24121)

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Overview

Product name

Anti-Urokinase antibody
See all Urokinase products (16) ...

Description

Rabbit polyclonal to Urokinase

Specificity

This antibody binds to human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa). This antibody is highly specific for urokinase, no crossreactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human whole plasma or plasma proteins do not interfere with its binding to urokinase.

Tested applications

IHC-P, ICC/IF, WB, Dot Blot, IHC-Fr, IHC-P, ELISA, RIAmore details

Cross reactivity

Reacts with

Human

Immunogen

Full length protein (Human). Extensively purified native HMW-tcuPA and LMW-scuPA (mixture 50/50), isolated from human urine.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is subjected to absorption with immobilized total human serum proteins to remove non-specific antibodies. The affinity purified antibody is obtained by binding to immobilized native human urokinase, followed by elution with acidic buffer, neutralization, dialysis, dispensing and lyophilization.

Clonality

Polyclonal

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Neurodegenerative disease
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Urokinase antibody (ab24121)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Urokinase antibody (ab24121) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab24121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.

Tissue specificity

Expressed in the prostate gland and prostate cancers.

Sequence similarities

Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.

Post-translational
modifications

Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.

Cellular localization

Secreted.

Target information above from: UniProt accessionP00749 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ATF antibody
  • ATF uPA antibody
  • Plasminogen activator antibody
  • Plasminogen activator urinary antibody
  • Plasminogen activator urokinase antibody
  • PLAU antibody
  • u PA antibody
  • U plasminogen activator antibody
  • U-plasminogen activator antibody
  • UPA antibody
  • URK antibody
  • UROK_HUMAN antibody
  • Urokinase plasminogen activator antibody
  • Urokinase type plasminogen activator antibody
  • Urokinase type plasminogen activator precursor antibody
  • Urokinase-type plasminogen activator chain B antibody
see all

Database links

Anti-Urokinase antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Urokinase antibody (ab24121)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Urokinase antibody (ab24121)

ab24121 at 1/200 dilution staining HMW-scuPA in human brain tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were formaldehyde fixed prior to blocking in 10% serum for 12 hours at room temperature and then incubated with ab24121, for 18 hours at 4°C. A goat monoclonal to rabbit Ig, diluted 1/50, was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Rachel Barker

See Abreview

References for Anti-Urokinase antibody (ab24121)

This product has been referenced in:

  • Lash GE  et al. Regulation of extravillous trophoblast invasion by uterine natural killer cells is dependent on gestational age. Hum Reprod 25:1137-45 (2010).Read more (PubMed: 20219775) »
  • Svenningsen P  et al. Plasmin in nephrotic urine activates the epithelial sodium channel. J Am Soc Nephrol 20:299-310 (2009).Read more (PubMed: 19073825) »

See all 4 publications for this product

Publishing research using ab24121? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"