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Urokinase type Plasminogen Activator Human ELISA Kit (ab108917)

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Overview

  • Product nameUrokinase type Plasminogen Activator Human ELISA KitSee all Urokinase type Plasminogen Activator kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Tissue
  • Assay typeSandwich
  • Sensitivity
    = 0.2 ng/ml
  • Range
    0.25 ng/ml - 8 ng/ml
  • Recovery

    101 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Urokinase-type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodeling in angiogeenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.

    Ab108917 Human Urokinase (uPA) ELISA kit is designed for detection of human uPA in plasma, serum, tissue, saliva, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures urokinase in less than 4 hours. A polyclonal antibody specific for uPA has been pre-coated onto a microplate. Urokinase in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for uPA, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionSpecifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • Tissue specificityExpressed in the prostate gland and prostate cancers.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P00749 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        ATFATF uPABDPLT5
        Plasminogen activatorPlasminogen activator urinaryPlasminogen activator urokinasePLAUQPDu PAU plasminogen activatorU-plasminogen activatoruPAURKUROK_HUMANUrokinase plasminogen activatorUrokinase type plasminogen activatorUrokinase type plasminogen activator precursorUrokinase-type plasminogen activator chain B
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108917 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Urokinase type Plasminogen Activator Human ELISA Kit images

      • ELISA - Urokinase type Plasminogen Activator Human ELISA Kit (ab108917)
        ELISA - Urokinase type Plasminogen Activator Human ELISA Kit (ab108917)

      Protocols

      References for Urokinase type Plasminogen Activator Human ELISA Kit (ab108917)

      ab108917 has not yet been referenced specifically in any publications.

      Publishing research using ab108917 ? Please let us know so that we can cite the reference in this datasheet

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      How much tissueand lysis buffer volume is needed for this kit.

      Thank you for your email.

      We generally recommend our customer using 400-500ul of lysis buffer per 5-10 mg of tissues however you may have to adjust it based upon your results.

      500ul lysis buffer for 1 million cells would be ideal.
      Read More

      How much tissueand lysis buffer volume is needed for this kit.

      Thank you for your email.

      We generally recommend our customer using 400-500ul of lysis buffer per 5-10 mg of tissues however you may have to adjust it based upon your results.

      500ul lysis buffer for 1 million cells would be ideal.
      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"