Anti-Uroplakin III antibody [SFI-1], prediluted (ab78197)
Key features and details
- Mouse monoclonal [SFI-1] to Uroplakin III, prediluted
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Uroplakin III antibody [SFI-1], prediluted
See all Uroplakin III primary antibodies -
Description
Mouse monoclonal [SFI-1] to Uroplakin III, prediluted -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to an internal region of Human Uroplakin III.
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Positive control
- Urothelial tissue, bladder.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.05% Proclin 300
Constituents: 0.363% Tris, 0.1% Tween, 0.58% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
SFI-1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Ready to use for 30 min at RT. |
Notes |
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IHC-P
1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Ready to use for 30 min at RT. |
Target
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Function
Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence. -
Tissue specificity
Expressed in ureter. -
Involvement in disease
Defects in UPK3A are a cause of renal adysplasia (RADYS) [MIM:191830]; also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy. -
Sequence similarities
Belongs to the uroplakin-3 family. -
Cellular localization
Endoplasmic reticulum membrane. Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER). - Information by UniProt
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Database links
- Entrez Gene: 7380 Human
- Omim: 611559 Human
- SwissProt: O75631 Human
- Unigene: 632787 Human
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Alternative names
- MGC119178 antibody
- OTTHUMP00000028951 antibody
- UP III antibody
see all
Images
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ab78197 staining Uroplakin III in human bladder by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
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ab78197 staining Uroplakin III in mouse bladder tissue sections. Tissue was fixed in formaldehyde and heat-mediated antigen retrieval was performed using citric buffer. Blocking was performed with 1% BSA for 10 minutes at 21ºC and ab78197 was used at 1/30 for 2 hours at 21ºC.
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ab78197 staining Uroplakin III in rat bladder tissue sections. Tissue was fixed in formaldehyde and heat-mediated antigen retrieval was performed using citric buffer. Blocking was performed with 1% BSA for 10 minutes at 21ºC and ab78197 was used at 1/30 for 2 hours at 21ºC.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab78197 has been referenced in 2 publications.
- Watanabe T et al. The Urinary Bladder is Rich in Glycosphingolipids Composed of Phytoceramides. J Lipid Res 63:100303 (2022). PubMed: 36441023
- Garzón I et al. Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells. Polymers (Basel) 13:N/A (2021). PubMed: 34068343