Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Uteroglobin antibody
See all Uteroglobin products (4) ...
Description
Rabbit polyclonal to Uteroglobin
Tested applications
IHC-P, ELISA, WB, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant rat uteroglobin His fusion protein.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Primary antibody notes
This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/1600.
ELISA: Use at an assay dependent concentration. PubMed: 21787397
WB: 1/5000. Predicted molecular weight: 10 kDa.
IHC-Fr: Use at an assay dependent dilution.
Target
Function
Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
Tissue specificity
Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
Sequence similarities
Belongs to the secretoglobin family.
Cellular localization
Secreted.
Target information above from: UniProt accessionP11684
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- blastokinin antibody
- CC10 antibody
- CC16 antibody
see all
Database links
- Entrez Gene: 7356 Human
- Entrez Gene: 22287 Mouse
- Entrez Gene: 25575 Rat
- Omim: 192020 Human
- SwissProt: P11684 Human
- SwissProt: Q06318 Mouse
- SwissProt: P17559 Rat
- Unigene: 523732 Human
see all
Anti-Uteroglobin antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Uteroglobin antibody (ab40873)
Adult mouse lung tissue.
Immunohistochemistry (Frozen sections) - Uteroglobin antibody (ab40873)
ab40873 at 1/1600 dilution staining Uteroglobin in mouse embryo tissue section by Immunohistochemistry (Frozen sections). Frozen tissue slides were fixed in 4% paraformaldehyde and antigen retrieval was performed for 10 minutes in microwave oven in 0.01 M citrate buffer pH6. ab40873 stains green in proximal lung section from wild type mouse embryo as shown in image.
Image from Nyeng P et. al., BMC Dev Biol. 2008; 8: 2 (Fig. 6D)
Western blot - Uteroglobin antibody (ab40873)
Anti-Uteroglobin antibody (ab40873) at 1/5000 dilution + Wild-type adult mouse lung homogenate was used.
Secondary
5% Dry milk, 1 hour at room temperature at 1/5000 dilution
Predicted band size : 10 kDa
Observed band size : 10 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Uteroglobin antibody (ab40873)
ab40873 staining Uteroglobin in human lung parenchyma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer. Samples were then blocked with 1% BSA, 0.1% Triton-X/ PBS for 15 minutes at 18°C followed by incubation with the primary antibody at a 1/500 dilution for 16 hours at 4°C. An HRP-conjugated pig anti-rabbit polyclonal was used as secondary antibody at a 1/100 dilution.
Image courtesy of an anonymous Abreview.
References for Anti-Uteroglobin antibody (ab40873)
This product has been referenced in:
- Maddens B et al. Chitinase-like proteins are candidate biomarkers for sepsis-induced acute kidney injury. Mol Cell Proteomics : (2012). WB; Mouse.Read more (PubMed: 22233884) »
- Bothwell SP et al. Mouse model for lowe syndrome/dent disease 2 renal tubulopathy. J Am Soc Nephrol 22:443-8 (2011). WB; Mouse.Read more (PubMed: 21183592) »
See all 7 publications for this product
Publishing research using ab40873? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"