![Western blot - V-type proton ATPase catalytic subunit A antibody [8B1F3] (ab113745) image](#V-type-proton-ATPase-catalytic-subunit-A-Primary-antibodies-ab113745-1.jpg)
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Product name
Anti-ATP6V1A antibody [8B1F3]
See all ATP6V1A products (5) ...
Description
Mouse monoclonal [8B1F3] to ATP6V1A
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Full length native protein (purified)
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
Clonality
Monoclonal
Clone number
8B1F3
Isotype
IgG2a
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Vacuole
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Western blot - V-type proton ATPase catalytic subunit A antibody [8B1F3] (ab113745)
![Western blot - V-type proton ATPase catalytic subunit A antibody [8B1F3] (ab113745) image](/ps/datasheet/images/113/ab113745/V-type-proton-ATPase-catalytic-subunit-A-Primary-antibodies-ab113745-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab113745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ICC/IF: Use a concentration of 20 µg/ml. heat induced antigen retrieval recommended
Target
Function
Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Tissue specificity
Present in all tissues analyzed.
Sequence similarities
Belongs to the ATPase alpha/beta chains family.
Target information above from: UniProt accessionP38606
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 70kDa subunit antibody
- ATP6A1 antibody
- ATP6V1A antibody
see all
Database links
- SwissProt: P17255 Saccharomyces cerevisiae
Anti-ATP6V1A antibody [8B1F3] images:
Western blot - V-type proton ATPase catalytic subunit A antibody [8B1F3] (ab113745)
Anti-ATP6V1A antibody [8B1F3] (ab113745) at 1 µg/ml + Yeast Whole Cell at 15 µg
Predicted band size : 69 kDa
References for Anti-ATP6V1A antibody [8B1F3] (ab113745)
ab113745 has not yet been referenced specifically in any publications.
Publishing research using ab113745? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"