Anti-V5 tag antibody (SureLight® Allophycocyanin) (ab72560)

Overview

  • Product name
    Anti-V5 tag antibody (SureLight® Allophycocyanin)
    See all V5 tag primary antibodies
  • Description
    Rabbit polyclonal to V5 tag (SureLight® Allophycocyanin)
  • Conjugation
    SureLight® Allophycocyanin. Ex: 652nm, Em: 657nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Immunogen

    V5 peptide conjugated to a carrier molecule.

  • General notes

    Excitation maximum: 650 nm Emission maximum: 660 nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    Preservative: 2mM Sodium Azide
    Constituents: 0.1% BSA, 150mM Sodium chloride, 100mM Sodium phosphate, pH 7.4
  • Concentration information loading...
  • Purity
    Size exclusion
  • Purification notes
    Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent dilution.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Images

  • Flow cytometry analysis of a recombinant (N-terminus V5-tag) transmembrane cell surface receptor in CHO cells using ab72560 at a 1/20 dilution (red). Black - Mouse IgG SureLight® Allophycocyanin isotype control.

  • Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated V5-tagged peptide and read after a 2 hour incubation at room temperature with ab72560.

References

ab72560 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Merci de nous avoir contactés.

Je viens d'être informé par le laboratoire que le volume recommandé pour une utilisation en cytométrie en flux est de 10-20 µl/test (1-2 µg/test) pour 100 µl d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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