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Anti-V5 tag antibody (SureLight® Allophycocyanin) (ab72560)

Overview

  • Product nameAnti-V5 tag antibody (SureLight® Allophycocyanin)See all V5 tag primary antibodies ...
  • Description
    Rabbit polyclonal to V5 tag (SureLight® Allophycocyanin)
  • ConjugationSureLight® Allophycocyanin
  • Conjugation notesExcitation maximum: 650 nm Emission maximum: 660 nm
  • Tested applicationsFlow Cyt more details
  • Immunogen

    V5 peptide conjugated to a carrier molecule.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark.
  • Storage bufferPreservative: 2mM Sodium Azide
    Constituents: 0.1% BSA, 150mM Sodium chloride, 100mM Sodium phosphate, pH 7.4
  • Concentration information loading...
  • PuritySize exclusion
  • Purification notesSize exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab72560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Flow Cyt: Use at an assay dependent dilution.

Target

  • RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Cellular localizationNot applicable
  • Alternative names
      GKPIPNPLLGLDST epitope tag antibodyGKPIPNPLLGLDST tag antibodyProtein Rev antibody
      Regulator of expression of viral proteins antibodyrev antibodyV5 epitope tag antibody
    see all

Anti-V5 tag antibody (SureLight® Allophycocyanin) images

  • Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated V5-tagged peptide and read after a 2 hour incubation at room temperature with ab72560.

References for Anti-V5 tag antibody (SureLight® Allophycocyanin) (ab72560)

ab72560 has not yet been referenced specifically in any publications.

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Je viens d'être informé par le laboratoire que le volume recommandé pour une utilisation en cytométrie en flux est de 10-20 µl/test (1-2 µg/test) pour 100 µl de p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"