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Products:Microbiology >> Interspecies Interaction >> Host Virus Interaction
Overview
Product name
Anti-VAC14 antibody
See all VAC14 products (2) ...
Description
Mouse polyclonal to VAC14
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length VAC14 protein (Human)
Positive control
Human liver tissue lysate. VAC14 transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
Target
Function
The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the VAC14 family.
Contains 6 HEAT repeats.
Domain
The C-terminal domain (residues 523-782) mediates homomeric interactions and is necessary for the formation and maintenance of the PI(3,5)P2 regulatory complex.
Cellular localization
Endosome membrane. Microsome membrane. Mainly associated with membranes of the late endocytic pathway.
Target information above from: UniProt accessionQ08AM6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ArPIKfyve antibody
- FLJ10305 antibody
- FLJ36622 antibody
see all
Database links
- Entrez Gene: 55697 Human
- Omim: 604632 Human
- SwissProt: Q08AM6 Human
- Unigene: 445061 Human
Anti-VAC14 antibody images:
Western blot - VAC14 antibody (ab67369)
Anti-VAC14 antibody (ab67369) at 1/500 dilution + Human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 88 kDa
Observed band size : 88 kDa
Western blot - VAC14 antibody (ab67369)
All lanes : Anti-VAC14 antibody (ab67369) at 1/500 dilution
Lane 1 : VAC14 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 88 kDa
Observed band size : 88 kDa
References for Anti-VAC14 antibody (ab67369)
ab67369 has not yet been referenced specifically in any publications.
Publishing research using ab67369? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"