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Western blot - VAMP1 antibody (ab3346)
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Immunocytochemistry/ Immunofluorescence - VAMP1 antibody (ab3346)
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Product Name
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VAMP1 antibody
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See all VAMP1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to VAMP1
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Immunogen
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Synthetic peptide: MSAPAQPPAEGTEGAAPG-C, corresponding to amino acids 1-18 of Rat VAMP1.
BLAST 'MSAPAQPPAEGTEGAAPG-C' with  or 
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Reacts with
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Hu, Ms, Rat, Dog
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Tested applications
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ICC/IF, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
Used at a dilution of 1/1000 for 48 hrs incubation on mouse adult retina cells (see Abreview for further information).
Western Blot: use at a concentration of 0.3 µg/ml.
Immunoprecipitation: assay dependent.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this antibody detects a 15 kDa protein representing VAMP 1 from rat brain whole protein extract.
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Cellular localization
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Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein
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Research areas
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Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs
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Relevance
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The Vesicle Associated Membrane Proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered. It has been shown that the extreme carboxy-terminus of VAMP1 is involved in subcellular vesicle targeting.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS, 1 mg/ml BSA, 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VAMP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VAMP1 antibody images, references, products related to ab3346 and other tools.
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VAMP1 antibody images:
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 Western blot - VAMP1 antibody (ab3346)
Western blot of VAMP-1 on rat brain extract using ab3346.
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Immunocytochemistry/ Immunofluorescence - VAMP1 antibody (ab3346)
ab3346 at a 1/1000 dilution staining mouse adult retina cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 48 hours. Bound antibody was detected using an Alexa Fluor ® 488 conjugated donkey anti rabbit antibody. Dilutions of 1/1000 through to 1/5000 were found to work. Staining in the outer plexiform layer was fainter than in the ganglion cell layer This image is courtesy of an anonymous Abreview
See Abreview
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References for VAMP1 antibody (ab3346)
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This product has been used in: (two most recent references)
Panaretakis T et al. Mechanisms of pre-apoptotic calreticulin exposure in immunogenic cell death. EMBO J 28:578-90 (2009). WB; Mouse. PubMed: 19165151
Nystuen AM et al. A null mutation in VAMP1/synaptobrevin is associated with neurological defects and prewean mortality in the lethal-wasting mouse mutant. Neurogenetics 8:1-10 (2007). PubMed: 17102983
If you publish research using ab3346 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to VAMP1
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VAMP1 antibody - more information
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