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Products:Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs
Overview
Product name
Anti-VAMP1 antibody
See all VAMP1 products (8) ...
Description
Rabbit polyclonal to VAMP1
Tested applications
ICC/IF, IP, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Dog, Human
Immunogen
Synthetic peptide: MSAPAQPPAEGTEGAAPG-C, corresponding to amino acids 1-18 of Rat VAMP1.
MSAPAQPPAE GTEGAAPG-C
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (Used at a dilution of 1/1000 for 48 hrs incubation on mouse adult retina cells (see Abreview for further information).)
IP: Use at an assay dependent dilution.
WB: Use a concentration of 0.3 µg/ml(By Western blot, this antibody detects a 15 kDa protein representing VAMP 1 from rat brain whole protein extract.)
IHC-P: Use a concentration of 2 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Tissue specificity
Nervous system, skeletal muscle and adipose tissue.
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localization
Mitochondrion outer membrane; Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome and Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome.
Target information above from: UniProt accessionP23763
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686H12131 antibody
- SYB 1 antibody
- SYB1 antibody
see all
Database links
- Entrez Gene: 6843 Human
- Entrez Gene: 22317 Mouse
- Entrez Gene: 25624 Rat
- Omim: 185880 Human
- SwissProt: P23763 Human
- SwissProt: Q62442 Mouse
- SwissProt: Q63666 Rat
- Unigene: 20021 Human
see all
Anti-VAMP1 antibody images:
Immunocytochemistry/ Immunofluorescence - VAMP1 antibody (ab3346)
ab3346 at a 1/1000 dilution staining mouse adult retina cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 48 hours. Bound antibody was detected using an Alexa Fluor ® 488 conjugated donkey anti rabbit antibody. Dilutions of 1/1000 through to 1/5000 were found to work.
Staining in the outer plexiform layer was fainter than in the ganglion cell layer
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VAMP1 antibody (ab3346)
ab3346 (2µg/ml) staining VAMP1 in human cerebral cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and some membrane staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-VAMP1 antibody (ab3346)
This product has been referenced in:
- Li Yet al. DHHC5 interacts with PDZ domain 3 of post-synaptic density-95 (PSD-95) protein and plays a role in learning and memory. J Biol Chem 285:13022-31 (2010). WB; Mouse.Read more (PubMed: 20178993) »
- Uthaiah RC & Hudspeth AJ Molecular anatomy of the hair cell's ribbon synapse. J Neurosci 30:12387-99 (2010).Read more (PubMed: 20844134) »
See all 4 publications for this product
Publishing research using ab3346? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"