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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab41324 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - VAMP1 antibody (ab41324)
All lanes : Anti-VAMP1 antibody (ab41324) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate - normal tissue
Lane 3 : Spinal Cord (Mouse) Tissue Lysate
Lane 4 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (PFA perfusion fixed frozen sections) - VAMP1 antibody (ab41324)
Immunohistochemistical detection of VAMP1 with ab41324 on rat brain PFA perfusion-fixed frozen sections. Primary antibody ab41324 diluted at 1/3000 and incubated for 18 hours at 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 (1/1000)Immunostaining obtained in the rat hippocampus using this antibody diluted 1:1000. Sections come from a perfused-fixed animal. The immunostaining was performed using the `free floating` technique, using direct fluorescence. The antibody stains synapses in many brain areas. The picture shows the staining taken at low power magnification, showing the staining in the hippocampus (top) and medium thalamus (bottom of the picture).
Sophie Pezet, CNRS, Paris, France
VAMP1 antibody for IHC-P in Rat (41324)
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